RNF31 Antibody
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Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we can ship products within 1-3 business days of receiving your order. Delivery times may vary depending on the purchasing method or location. Please contact your local distributor for specific delivery details.
Synonyms
E3 ubiquitin protein ligase RNF31 antibody; FLJ10111 antibody; FLJ23501 antibody; HOIL 1L interacting protein antibody; HOIL-1-interacting protein antibody; HOIL1L interacting protein antibody; HOIP antibody; RING finger protein 31 antibody; RNF31 antibody; RNF31_HUMAN antibody; ZIBRA antibody; Zinc in between ring finger ubiquitin associated domain antibody; Zinc in-between-RING-finger ubiquitin-associated domain protein antibody
Target Names
Uniprot No.
Target Background
Function
RNF31 is an E3 ubiquitin-protein ligase component of the LUBAC complex. This complex is responsible for conjugating linear ('Met-1'-linked) polyubiquitin chains to substrates, playing a crucial role in NF-kappa-B activation and inflammation regulation. LUBAC specifically attaches linear polyubiquitin to IKBKG and RIPK1, thereby activating the canonical NF-kappa-B and JNK signaling pathways. The linear ubiquitination process mediated by LUBAC prevents TNF-induced cell death and consequently suppresses inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) upon polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3. This recruitment enables LUBAC to conjugate linear polyubiquitin to IKBKG and potentially other components, contributing to the stability of the complex. In conjunction with OTULIN, LUBAC regulates the canonical Wnt signaling pathway during angiogenesis. RNF31 is essential for linear ubiquitination of BCL10, ultimately promoting TCR-induced NF-kappa-B activation. Additionally, RNF31 binds polyubiquitin chains of various linkage types.
Gene References Into Functions
- The binding of SHARPIN or HOIL-1L facilitates the E2 loading of HOIP. PMID: 28978479
- SPATA2 has been identified as a previously unrecognized factor in the linear ubiquitin chain assembly complex-dependent signaling pathways. It acts as an adaptor between HOIP and CYLD, enabling the recruitment of CYLD to signaling complexes. PMID: 27545878
- This review highlights recent discoveries on RNF31's roles in nuclear factor modifications, breast cancer progression, and potential therapeutic inhibitors targeting RNF31. [review] PMID: 27460922
- Research has established SPATA2 as a high-affinity binding partner of CYLD and HOIP, and a regulatory component of linear ubiquitin chain assembly complex-mediated NF-kappaB signaling. PMID: 27591049
- LUBAC components exert control over TLR3-mediated innate immunity, thereby preventing the development of immunodeficiency and autoinflammation. PMID: 27810922
- A study revealed that RNF31 is cleaved under apoptotic conditions through various stimulations, shedding light on a novel regulatory loop between cell death and survival signals. PMID: 27669734
- These findings indicate that caspase-mediated cleavage of HOIP divides critical functional regions, regulating linear (de)ubiquitination of substrates upon apoptosis. PMID: 28189684
- RNF31 has been observed to decrease p53 stability. Depletion of RNF31 resulted in cell cycle arrest and cisplatin-induced apoptosis in a p53-dependent manner. RNF31 associates with the p53/MDM2 complex, facilitating p53 polyubiquitination and degradation by stabilizing MDM2. PMID: 26148235
- This study describes a novel posttranslational regulation of the HOIP-containing linear-ubiquitin-chain assembly complex (LUBAC)-mediated linear ubiquitination. This regulation is crucial for specifically directing TLR4-mediated NF-kappaB activation. PMID: 26578682
- These findings collectively suggest an oncogene role for RNF31 in PCa progression, which can be regulated by miR-503, implying that RNF31 could serve as a potential prognostic biomarker and therapeutic target for PCa. PMID: 26231797
- We identified BCL10 as a bona fide target of BCR-induced linear ubiquitylation and demonstrated a significant role for the linear ubiquitin ligase HOIP in BCR-induced phosphorylation. PMID: 26038114
- The crystal structure of a HOIP/E2~ubiquitin complex reveals the RBR E3 ligase mechanism and its regulation. PMID: 26789245
- Human HOIP is essential for the assembly and function of LUBAC, which includes HOIL-1, and for various processes governing inflammation and immunity in both hematopoietic and nonhematopoietic cells. PMID: 26008899
- Two rare germline polymorphisms affecting the LUBAC subunit RNF31 were identified and enriched among patients with activated B cell-like subtype of diffuse large B-cell lymphoma. PMID: 24491438
- RNF31, through stabilizing ESR1 levels, controls the transcription of estrogen-dependent genes linked to breast cancer cell proliferation. PMID: 24441041
- Phosphorylation of OTULIN prevents HOIP binding, whereas unphosphorylated OTULIN is part of the endogenous LUBAC complex. PMID: 24726323
- HOIP binding to OTULIN is required for the recruitment of OTULIN to the TNF receptor complex. PMID: 24726327
- Target specificity toward NEMO is determined by multiple LUBAC components, while linear ubiquitin chain elongation is achieved through a specific interplay between HOIP and ubiquitin. PMID: 24030825
- The crystal structure of the catalytic core of HOIP in its apo form and in complex with ubiquitin has been determined. PMID: 24141947
- The HOIP component of the linear ubiquitin assembly complex catalyzes the formation of M1-polyubiquitin chains in response to interleukin-1. PMID: 23986494
- Research has elucidated how HOIP combines a general RING-IBR-RING ubiquitin ligase mechanism with unique, 'Linear ubiquitin chain Determining Domain'-dependent specificity for producing linear ubiquitin chains. PMID: 22863777
- Analysis of differentially expressed genes indicates a systemic role for SF-1 in the adrenal cortex affecting differentiation, proliferation, and steroidogenesis, establishing RNF31 as a critical regulator of adrenal steroidogenesis. PMID: 22427816
- The study highlights the versatility and specificity of protein-protein interactions involving the Ub-like domain (UBL) of HOIL-1L/the Ub-associated domain (UBA) of HOIP and their cognate proteins. PMID: 22430200
- ZIBRA was identified and its genomic organization, regulation, and expression were studied in breast cancer cells. PMID: 15093743
- RNF31 functions to stabilize DAX-1; RNF31 appears to be required for DAX-1 to repress transcription. PMID: 19237537
Database Links
Subcellular Location
Cytoplasm.
Tissue Specificity
Expressed in both normal and transformed breast epithelial cell lines.
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