RNH203 Antibody

RNH203 in the RNase H2 Complex

The RNase H2 complex in yeast comprises three subunits: Rnh201 (catalytic subunit), Rnh202, and Rnh203. RNH203 stabilizes the heterotrimer and facilitates substrate recognition. Key functions include:

• Ribonucleotide Excision Repair (RER): RNase H2 initiates RER by cleaving at single rNMPs embedded in DNA, followed by repair synthesis and ligation .

• RNA/DNA Hybrid Processing: RNase H2 degrades RNA primers during Okazaki fragment maturation and resolves transcription-associated R-loops .

Research Applications of RNH203 Antibody

While the provided sources do not explicitly describe commercial RNH203 antibodies, studies on homologous systems (e.g., human RNASEH2C ) and yeast RNase H2 mutants inform its potential uses:

• Western Blotting: Detecting RNH203 protein levels in yeast strains under replication stress or DNA damage .

• Localization Studies: Mapping RNH203 recruitment to replication forks or DNA damage foci via immunofluorescence .

• Functional Analysis: Validating RNH203 knockout or separation-of-function mutants (e.g., Rnh203-RED, which retains RNA/DNA hybrid activity but lacks rNMP cleavage) .

Table 1: Enzymatic Activity of RNase H2 Variants

• Genome Instability: Yeast lacking RNH203 exhibit elevated mutation rates, replication stress, and sensitivity to hydroxyurea (HU) or methyl methanesulfonate (MMS) .

• Disease Relevance: Mutations in human RNASEH2 homologs are linked to Aicardi-Goutières Syndrome (AGS) and cancer, underscoring conserved roles in genome maintenance .

Technical Considerations

• Specificity: Polyclonal antibodies against RNASEH2C (human homolog) show cross-reactivity with yeast RNH203 in some contexts, but validation via knockout controls is critical .

• Functional Assays: RNH203 antibody aids in studying RNase H2’s interaction with PCNA, a replication fork processivity factor .

Future Directions

Research leveraging RNH203 antibodies could explore:

• Temporal regulation of RER during the cell cycle.

• Mechanisms of RNase H2 recruitment to stressed replication forks.

• Links between rNMP accumulation, inflammation, and cancer .