RNPEP Antibody
Description
Overview of RNPEP Antibody
RNPEP antibodies are immunoreagents designed to detect arginyl aminopeptidase (aminopeptidase B), encoded by the RNPEP gene. These antibodies facilitate the study of RNPEP's enzymatic activity, localization, and expression across tissues. Key characteristics include:
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Host/Isotype: Primarily rabbit-derived IgG polyclonal antibodies .
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Immunogen: Typically recombinant proteins or fusion peptides (e.g., Proteintech’s Ag6526 fusion protein) .
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Reactivity: Confirmed in human, mouse, and rat samples, with cross-reactivity validation in cell lines (e.g., HeLa, A549) and tissues (liver, lung cancer) .
Tested Applications and Dilutions
RNPEP antibodies are validated for multiple techniques:
Disease Associations
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Cancer Metastasis: RNPEP in tumor exosomes promotes lung metastasis in liver cancer by activating cancer-associated fibroblasts .
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Neurological Disorders: Reduced RNPEP levels in Mecp2-deficient mice (Rett syndrome model) suggest its role in cortical protein regulation .
Protocol Optimization
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Antigen Retrieval: For IHC, TE buffer (pH 9.0) or citrate buffer (pH 6.0) enhances detection in formalin-fixed tissues .
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Validation: Specificity confirmed via protein arrays (Novus Biologicals) and knockdown/knockout models .
Validation and Quality Control
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Specificity: Verified using siRNA knockdown and immunoblotting .
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Cross-Reactivity: No significant binding to 383 non-target proteins in array testing .
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Batch Consistency: Antigen-affinity purification ensures high lot-to-lot reproducibility .
Limitations and Considerations
Product Specs
Target Background
- Angiotensin II, aminopeptidases-A, B, M, and IRAP were found in the jejunal mucosa. PMID: 26311161
- Cytosolic peptidases, such as puromycin-sensitive aminopeptidase (PSA), aminopeptidase B (APB), and pyroglutamyl-peptidase I (PGI), might play a role in colorectal carcinogenesis. PMID: 26078706
- These findings suggest a potential role for plasma peptidases in the pathogenesis of first-episode psychosis, indicating that aminopeptidase activity might serve as a prognostic indicator. PMID: 25997998
- The Y229H substitution in aminopeptidase B (Ap-B) preserves enzyme activity, suggesting the importance of hydrogen bonds in its function. PMID: 25530263
- The activities of aminopeptidases N and B, along with insulin-regulated aminopeptidase, might be useful non-invasive biomarkers for Alzheimer's disease, even in its early stages. PMID: 23500679
- These data strongly indicate that the Asp 405 residue of aminopeptidase B is involved in substrate binding via an interaction with the P1 amino group of the substrate's side chain. PMID: 16981702
