ROBO3 Antibody
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Description
Mechanism of Action in Neural Development
ROBO3 regulates midline crossing of commissural axons in the spinal cord and hindbrain. Unlike other ROBO proteins, it facilitates midline crossing by antagonizing SLIT2-ROBO1 repulsion:
| Interaction | Effect |
|---|---|
| ROBO3-ROBO1 binding | Blocks SLIT2-mediated repulsion |
| ROBO3-SLIT2 binding | Nonproductive interaction (Form B only) |
Applications in Research
ROBO3 antibodies are widely used in:
Neural Development
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ROBO3 guides commissural axons by modulating Netrin-1 and VEGF/Flk1 signaling .
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Embryonic mouse studies show ROBO3 expression in the forebrain and spinal cord, peaking at E11.5 .
Cancer Biology
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ROBO3s overexpression in basal-like breast cancer (BLBC) correlates with:
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Prognostic value: High ROBO3s levels predict reduced survival in BLBC patients .
Future Directions
Product Specs
Buffer
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. Store at -20°C. Avoid freeze / thaw cycles.
Lead Time
Typically, we can ship your orders within 1-3 business days after receiving them. Delivery times may vary depending on the purchasing method or location. Please contact your local distributors for specific delivery times.
Synonyms
FLJ21044 antibody; HGPPS antibody; HGPS antibody; RB inhibiting gene 1 antibody; Rbig 1 antibody; Rbig1 antibody; Retinoblastoma inhibiting gene 1 antibody; Rig 1 antibody; Rig1 antibody; Robo 3 antibody; Robo3 antibody; Robo3 protein antibody; ROBO3_HUMAN antibody; Roundabout axon guidance receptor homolog 3 antibody; Roundabout homolog 3 antibody; Roundabout like protein 3 antibody; Roundabout, axon guidance receptor, homolog 3 (Drosophila) antibody; Roundabout-like protein 3 antibody
Target Names
ROBO3
Uniprot No.
Target Background
Function
ROBO3 is believed to be involved in axonal navigation at the ventral midline of the neural tube during neural development. In spinal cord development, it plays a role in guiding commissural axons, likely by preventing premature sensitivity to Slit proteins, thus inhibiting Slit signaling through ROBO1. This function is essential for hindbrain axon midline crossing.
Gene References Into Functions
- We identified a novel homozygous mutation, p.R842* (c.2524C>T), resulting in a premature stop codon. This mutation is considered disease-causing. (PMID: 27267957)
- Our findings confirmed that ROBO3 expression increases with clinical grade, while miR-383 expression is inversely correlated with ROBO3 expression. (PMID: 26070964)
- Human AKAP79-anchored PKC selectively phosphorylates the Robo3.1 receptor subtype on serine 1330. (PMID: 25882844)
- We documented the case of a 10-month-old girl with cross-fixation and inability to abduct who was genetically diagnosed with horizontal gaze palsy with progressive scoliosis (recessive ROBO3 mutations). (PMID: 24969490)
- Robo3.1A may prevent Slit responsiveness by recruiting Robo1/2 into a late endosome- and lysosome-dependent degradation pathway. (PMID: 24936616)
- The novel ROBO3 mutation identified in this family may be among the most deleterious. (PMID: 21592015)
- This patient exhibited clinical and neuroimaging characteristics considered pathognomonic of horizontal gaze palsy and progressive scoliosis but did not have ROBO3 mutations. (PMID: 21510772)
- Three novel ROBO3 mutations were identified in consanguineous patients with horizontal gaze palsy and progressive scoliosis. (PMID: 21850172)
- Slit3 inhibits Robo3-induced invasion of synovial fibroblasts in rheumatoid arthritis. (PMID: 20298552)
- Mutations in the ROBO3 gene were identified in patients affected with horizontal gaze palsy with progressive scoliosis (HGPPS). ROBO3 is required for hindbrain axon midline crossing. (PMID: 15105459)
- The major clinical characteristics of patients with mutated ROBO3 were horizontal gaze palsy, progressive scoliosis, and brainstem malformations. (PMID: 15824346)
- We describe and compare two human Robo3 isoforms, Robo3A and Robo3B, which differ by the insertion of 26 amino acids at the N-terminus. These forms appear to be evolutionarily conserved. (PMID: 16226035)
- The incidence of scoliosis in individuals carrying heterozygous ROBO3 mutations may be greater than in the general population. (PMID: 16525029)
- No evidence was found for an association between Gilles de la Tourette Syndrome and either the ROBO3 gene. Thus, this gene is unlikely to be a susceptibility gene contributing to GTS on 11q24. (PMID: 17671968)
- Four SNPs of ROBO3 showed associations with autism. (PMID: 18270976)
- This study identified five novel homozygous ROBO3 mutations (four missense mutations and one base deletion) distributed throughout the extracellular domain of the gene in consanguineous families with horizontal gaze palsy and progressive scoliosis. (PMID: 18829051)
- This is the second reported patient with synergistic convergence and the first associated with a documented pathologic genotype. (PMID: 19041479)
- Mutations in the ROBO3 gene, which shares homology with roundabout genes important in axon guidance in developing Drosophila, zebrafish, and mouse, were identified. (PMID: 15105459)
Database Links
Involvement In Disease
Gaze palsy, familial horizontal, with progressive scoliosis, 1 (HGPPS1)
Protein Families
Immunoglobulin superfamily, ROBO family
Subcellular Location
Membrane; Single-pass type I membrane protein.
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