RPS15 Antibody
Description
Introduction to RPS15 Antibody
RPS15 (ribosomal protein S15) is a 17 kDa protein component of the 40S ribosomal subunit, essential for mRNA translation and ribosome biogenesis . The RPS15 Antibody (e.g., Proteintech 14957-1-AP) is a rabbit-derived IgG antibody validated for detecting RPS15 in human, mouse, and rat samples across multiple applications, including Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF/ICC) .
Table 2: Recommended Dilutions
| Application | Dilution Range |
|---|---|
| Western Blot | 1:500 – 1:2,400 |
| IHC | 1:400 – 1:1,600 |
| IF/ICC | 1:20 – 1:200 |
Oncogenic Role in Esophageal Squamous Cell Carcinoma (ESCC)
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In Vitro Effects:
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Mechanistic Insights:
Table 3: Functional Impact of RPS15 Modulation in ESCC
| Cell Line | RPS15 Modification | Phenotypic Change | Key Pathways Affected |
|---|---|---|---|
| KYSE30/KYSE450 | Overexpression | ↑ Migration, invasion, proliferation | E2F, p38 MAPK |
| KYSE150/KYSE510 | Knockout | ↓ Metastasis, ↓ tumor growth in vivo | Ribosome biogenesis |
Applications in Biomedical Research
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Diagnostic Use: Detects RPS15 in human cancers (e.g., lymphoma, liver cancer) and normal tissues (liver, brain) .
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Therapeutic Potential: Identified as a target for anticancer strategies due to its role in ESCC progression .
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Senescence Studies: Knockdown of RPS15 in mouse embryonic fibroblasts (MEFs) disrupts ribosome assembly, linking it to cellular aging .
Product Specs
Customer Reviews
Applications : Western blotting
Sample type: cells
Review: The markers of apoptosis, such as pro‐caspase3, p53, and cleaved Poly (ADP‐ribose) polymer‐ase (PARP), and ribosomal biogenesis marker, viz. 40S ribosome protein 15 (RPS15), were induced by the ectopic expression of nuclear αSyn. The densities of protein bands assessed using Western blotting were estimated and compared by the normalization with the density of β‐tubulin band. We used one‐way ANOVA with Bonferroni’s post hoc test. Data are represented as the mean ±SEM.
