RPS28 Antibody

Applications and Validation Data

RPS28 antibodies are widely used in:

Key Applications

• Western Blot (WB): Detects RPS28 at ~8 kDa in lysates from HepG2, HEK-293T, and NIH/3T3 cells .

• Immunohistochemistry (IHC): Validated in human pancreatic cancer tissues with recommended dilutions of 1:20–1:200 .

• Immunofluorescence (IF): Localizes RPS28 in cytoplasmic and nuclear compartments .

• ELISA/Immunoprecipitation (IP): Confirms specificity via genetic knockdown controls .

Recommended Dilutions

3.1. Cancer Research

• Osteosarcoma Prognosis: High RPS28 expression correlates with poor survival. Knockdown inhibits tumor proliferation and metastasis via MAPK pathway modulation .

• Therapeutic Target: RPS28 antibodies identify overexpression in colorectal and hepatocellular carcinomas, linking it to oncogenic ribosome activity .

3.2. Immunology

• Antigen Presentation: RPS28 knockdown increases HLA-A2 surface expression by enhancing peptide supply, independent of TAP transporters .

3.3. Cellular Biology

• Stress Granule Dynamics: Yeast RPS28B mRNA scaffolds processing body (PB) assembly, with rps28bΔ mutants showing defective PB formation under stress .

Clinical and Mechanistic Insights

• Disease Association: Mutations in RPS28 cause Diamond-Blackfan Anemia 15, a ribosomopathy with mandibulofacial dysostosis .

• Regulatory Role: A tRNA-derived small RNA (LeuCAG3′tsRNA) binds Rps28 mRNA to regulate its translation, highlighting post-transcriptional control mechanisms .