RRA2 Antibody

ARR-002 (Alomone Labs)

• Epitope: Human RyR2 (specific sequence not disclosed).

• Immunohistochemical Staining:

  • Cardiac Muscle: Specific staining in cardiomyocytes; no signal in vascular smooth muscle cells .

  • Brain: Detection in mouse cerebellum and hippocampal neurons .

• Western Blot: Confirmed reactivity with human, rat, and mouse lysates .

EPR26288-70 (Abcam)

• Epitope: Rabbit monoclonal targeting a conserved region of RyR2.

• Immunohistochemical Applications:

  • Human Cardiac Muscle: Cytoplasmic staining in paraffin-embedded sections .

  • Mouse Liver: Detection of RyR2 in frozen sections using fluorescent labeling .

Research Findings and Functional Insights

RyR2 antibodies have been instrumental in elucidating channel localization and its role in calcium signaling. Key studies include:

**3.1. Cardiac Muscle Function

• Excitation-Contraction Coupling: RyR2 antibodies confirm the protein’s localization to SR membranes, enabling calcium release during systole .

• Pathological Relevance: Dysregulation of RyR2 is implicated in arrhythmias and heart failure. Antibodies aid in studying mutations or post-translational modifications affecting channel gating .

**3.2. Neurological Applications

• Brain Expression: RyR2 is detected in cerebellar neurons and hippocampal cells, suggesting roles in neuronal calcium signaling and synaptic plasticity .

**3.3. Comparative Analysis of Antibody Performance

**4.1. Optimized Protocols

• Immunohistochemistry:

  • ARR-002: Paraffin-embedded sections require antigen retrieval (e.g., Tris-EDTA buffer) .

  • EPR26288-70: Heat-mediated antigen retrieval enhances signal in formalin-fixed tissues .

• Western Blot:

  • ARR-002: Effective with SDS-PAGE-separated lysates; detects a ~135 kDa band .

**4.2. Limitations

• Species-Specificity: Most antibodies lack reactivity with non-mammalian models.

• Epitope Accessibility: Fixed tissues may require optimization for antibody binding.