RRN7 Antibody

Structure and Function of Rrn7

Rrn7 is a 66-kDa protein that forms part of the Pol I Core Factor, a multiprotein complex essential for initiating transcription at rDNA promoters. Its structural domains include:

• Zinc ribbon domain (ZR) and cyclin fold repeats in the N-terminal region, which mediate protein-protein interactions.

• Unstructured B-reader/B-linker (BR/BL) regions, analogous to those in TFIIB-like factors, facilitating transcription initiation.

• A large C-terminal domain (CTD) with Pol I-specific regulatory functions .

Rrn7 interacts with Rrn6 and Rrn11 to form the Core Factor, which recruits TBP and Pol I to rDNA promoters. Mutational studies confirm that Rrn7 is indispensable for rDNA transcription in yeast .

Applications of RRN7 Antibody

The RRN7 antibody is employed in various experimental setups to study Rrn7’s biological roles:

Research Findings and Functional Insights

• Transcription Initiation: Rrn7, via its TFIIB-like domains, facilitates Pol I binding and promoter melting, enabling transcription initiation .

• Cross-Talk Between Pol I and Pol II: Rrn7 binds HomolD box promoters in ribosomal protein genes, linking rDNA and ribosomal gene transcription .

• Evolutionary Conservation: Human TAF1B, a homolog of Rrn7, performs analogous functions in Pol I transcription .