RPS16A Antibody

Antibody Characteristics

Commercial RPS16 antibodies exhibit consistent performance across platforms, as demonstrated by these specifications from leading providers:

Sources:

Biological Functions of RPS16

RPS16 contributes to both ribosomal and non-ribosomal pathways:

Ribosomal Role

• Essential for rRNA processing and translational control via RNA binding .

• Part of the small subunit (SSU) processome, critical for ribosome assembly .

Extra-Ribosomal Roles

Cancer Biology

• HCC Progression: USP1 stabilizes RPS16 by removing K48-linked ubiquitination, promoting HCC cell proliferation and metastasis. Clinically, high USP1/RPS16 expression correlates with poor survival (HR = 2.34, p < 0.001) .

• Prostate Cancer: RPS16 overexpression drives tumor growth via EMT (↓E-cadherin, ↑N-cadherin/Snail) and c-Myc stabilization .

• Therapeutic Target: Knockdown of RPS16 in HepG2 cells induces G0/G1 arrest and apoptosis, confirming its pro-tumorigenic role .

Viral Pathogenesis

• RPS16 interacts with Epstein-Barr virus LMP1, enhancing its stability and promoting oncogenesis .

• In plant models, RPS16 homologs inhibit viral replication by suppressing RNA silencing .

Clinical and Experimental Applications

• Diagnostic Use: IHC staining with RPS16 antibodies shows strong cytoplasmic positivity in breast carcinoma and neuronal tissues .

• Functional Studies: siRNA-mediated RPS16 knockdown reduces HCC xenograft growth by 60% in murine models .

• Biomarker Potential: Overexpression in prostate (AUC = 0.82) and gastric cancers (HR = 1.7, p = 0.003) highlights prognostic utility .

Validation and Protocols

• Western Blot: Consistent detection at 16 kDa in HeLa, MCF-7, and liver lysates .

• IHC Optimization: Antigen retrieval with citrate (pH 6.0) or TE buffer (pH 9.0) improves signal in FFPE tissues .

• Cross-Reactivity: Mouse-specific antibodies (e.g., Boster Bio A05230) enable translational research .