RPS25B Antibody

Overview of RPS25 Antibodies

RPS25 antibodies are tools designed to detect the 40S ribosomal protein S25, which plays roles in viral replication, p53-mediated apoptosis, and non-canonical translation mechanisms like repeat-associated non-AUG (RAN) translation . These antibodies are validated for applications including Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA .

Role in Viral Replication and Cancer

• Viral Mechanisms: RPS25 is critical for hepatitis C virus (HCV) and Dicistroviridae replication . Knockout reduces HCV replication efficiency .

• Cancer Associations: Overexpressed in adriamycin-resistant leukemia cells and linked to MDM2-p53 signaling . Detected in breast cancer, liver, and lymph node tissues .

Neurodegenerative Diseases

• C9orf72 ALS/FTD: RPS25 facilitates RAN translation of toxic dipeptide repeats (e.g., poly-GP, poly-GA) from GGGGCC repeats. Knockout reduces poly-GP levels by 50% and rescues toxicity in Drosophila and patient-derived motor neurons .

• Therapeutic Target: Antisense oligonucleotides (ASOs) against RPS25 lower poly(GR) and poly(PR) foci in ALS models .

Technical Validation Data

• Western Blot: Detected in A549, HeLa, K-562, and MCF-7 cell lines .

• IHC: Strong staining in human breast carcinoma and liver tissues .

• Knockout Models: RPS25 KO cells show unchanged global translation but reduced polysome-associated GGGGCC RNA .

Discussion of Limitations and Gaps

• No studies in the provided sources address a putative RPS25B isoform.

• Most antibodies are validated for human use; cross-reactivity with other species (e.g., mouse, rat) is limited .

• Functional studies focus on viral and neurodegenerative contexts; cancer-related mechanisms remain underexplored.