RPS7B Antibody

Introduction to RPS7 Antibodies

RPS7 antibodies target the 40S ribosomal protein S7, a component of the small ribosomal subunit critical for protein synthesis. These antibodies are primarily used to study ribosomal biogenesis, developmental disorders, and diseases linked to ribosomal dysfunction .

Notes:

• All antibodies detect endogenous RPS7 at ~22 kDa .

• Optimal dilutions vary by application (e.g., 1:50–1:200 for IHC-P) .

4.1. Immunohistochemistry (IHC)

• AKI Studies: Anti-RPS7 antibody (1:500 dilution) used in cisplatin-treated kidney tissues showed upregulated RPS7 expression correlating with ferroptosis .

• Cellular Localization: RPS7 localizes to the cytoplasm and ribosomes, confirmed via subcellular fractionation .

4.2. Functional Studies

• Ribosome Assembly: Mutant RPS7 proteins (Mtu and Zma) incorporate into ribosomes but disrupt rRNA processing .

• Behavioral Phenotypes: Rps7 haploinsufficiency in mice causes working memory deficits, implicating RPS7 in CNS development .

Limitations and Future Directions

• Species-Specific Differences: Murine Rps7 mutations do not replicate human DBA phenotypes, suggesting divergent roles across species .

• Therapeutic Potential: Targeting RPS7 in AKI requires further validation of ferroptosis pathways .