rsrIM Antibody

Overview of rsrIM Antibody

The rsrIM Antibody is a polyclonal rabbit-derived antibody targeting Modification methylase RsrI (EC 2.1.1.72), a bacterial enzyme responsible for methylating adenine in the double-stranded DNA sequence GAATTC. This methylation protects DNA from cleavage by the restriction enzyme RsrI, making the antibody a critical tool in molecular biology and epigenetics research .

Enzyme Activity Studies

The rsrIM Antibody is primarily used to detect the methylase enzyme in assays such as:

• ELISA: Quantitative analysis of RsrI methylation activity .

• Western Blot: Immunoblot validation of enzyme expression in bacterial lysates .

Epitope Recognition

The antibody binds to the catalytic domain of RsrI, which recognizes the sequence GAATTC and methylates adenine residues. This specificity is critical for studying DNA-protein interactions in restriction-modification systems .

Antibody Characterization

While the rsrIM Antibody meets basic validation criteria (e.g., target binding), adherence to rigorous characterization standards is essential for reproducibility. Key considerations include:

• Specificity: Ensure no cross-reactivity with non-target proteins (e.g., other methyltransferases) .

• Performance: Validate under experimental conditions (e.g., Western Blot buffer compatibility) .

Limitations

• Species Reactivity: Restricted to Rhodobacter sphaeroides, limiting utility in cross-species studies .

• Conjugate Variability: HRP, FITC, or unconjugated formats require distinct protocols (e.g., ELISA vs. fluorescence microscopy) .