RSU1 Antibody

Basic Research Questions

• What is RSU1 and why are antibodies against it important for research?

  RSU1 (Ras Suppressor protein 1) is a leucine-rich repeat (LRR)-containing protein originally identified based on its ability to inhibit v-Ras transformation . It's involved in the Ras signal transduction pathway, growth inhibition, and nerve-growth factor induced differentiation processes . RSU1 antibodies are crucial for studying this protein's role in focal adhesion formation, cell motility, and cancer progression .

  RSU1 interacts with PINCH-1, connecting it to Integrin Linked Kinase (ILK), alpha-parvin (PARVA), and the actin cytoskeleton . This positions RSU1 at a critical junction between extracellular matrix adhesion and intracellular signaling, making it an important research target in both normal cellular processes and disease states.

• What are the key specifications to consider when selecting an RSU1 antibody?

  When selecting an RSU1 antibody, researchers should consider several critical parameters:

  Parameter	Considerations
  Host species	Mouse monoclonal and rabbit polyclonal options are available
  Reactivity	Verify reactivity with target species (human, mouse, rat, etc.)
  Applications	Common applications include WB, IHC, IP, ELISA, ICC, IF
  Epitope region	Some target full-length protein, others target specific regions (C-terminal)
  Clonality	Monoclonal (e.g., 1C6, 3F10) vs polyclonal options
  Calculated MW	~31-32 kDa (verify with observed MW)

  For research requiring high specificity, monoclonal antibodies like clone 1C6 may be preferred, while polyclonal antibodies might offer higher sensitivity in certain applications .

• How should RSU1 antibodies be stored and handled for optimal results?

  Proper storage and handling of RSU1 antibodies is crucial for maintaining their performance:

  • Store at -20°C or lower

  • Aliquot to avoid repeated freezing and thawing cycles which can degrade antibody quality

  • Most RSU1 antibodies are supplied in phosphate buffered saline (pH 7.2-7.3) with stabilizers like glycerol (50%) and sometimes sodium azide (0.02%)

  • For long-term storage (>1 year), maintain at -20°C in aliquots

  • Prior to use, thaw antibodies on ice and centrifuge briefly to collect contents at the bottom of the tube

  • For optimal results in Western blot applications, dilutions typically range from 1:200-1:1000 depending on the specific antibody and sample type

• What are the recommended dilutions for different applications of RSU1 antibodies?

  Optimal dilutions vary by application and specific antibody:

  Application	Typical Dilution Range	Notes
  Western Blot (WB)	1:200-1:1000	Sample-dependent; may require optimization
  Immunoprecipitation (IP)	0.5-4.0 μg per 1.0-3.0 mg of total protein lysate	For pull-down experiments
  Immunohistochemistry (IHC)	Varies by antibody	Check product-specific recommendations
  ELISA	Varies by antibody	Typically higher dilutions than WB

  It's recommended to perform titration experiments to determine optimal dilutions for each specific experimental system . Published literature often includes detailed methods sections that can provide guidance for specific research applications .

Research Methodology Notes

When designing experiments using RSU1 antibodies, researchers should consider:

• The specific isoform being targeted and whether the antibody can distinguish between RSU-1L and RSU-1-X1

• The potential compensatory mechanisms between RSU1 isoforms that may affect experimental outcomes

• The interplay between RSU1 and the PINCH-ILK-PARVA complex in different cellular contexts

• The impact of experimental conditions (2D vs 3D culture, ECM stiffness) on RSU1 expression and function

• The need for multiple complementary techniques (Western blot, immunofluorescence, co-IP) to fully characterize RSU1 biology