RTP1 Antibody

Definition and Biological Role of RTP1

RTP1 is a membrane-associated protein that facilitates the functional cell surface expression of olfactory receptors, a subclass of G protein-coupled receptors (GPCRs) . It ensures proper localization of these receptors, enabling odorant detection and signal transduction. RTP1 is conserved across humans, mice, and rats, with UniProt IDs P59025 (human) and Q8C8C1 (mouse) .

RTP1 Antibody Characteristics

RTP1 antibodies are polyclonal or monoclonal reagents targeting specific epitopes of the protein. Key features include:

Target Epitopes

• Most antibodies recognize the C-terminal region (e.g., amino acids 168–197 in humans) .

• Immunogens often include synthetic peptides conjugated to carriers like KLH .

Key Notes

• Cross-reactivity: Most antibodies are validated for human, mouse, and rat samples .

• Storage: Stable at -20°C in PBS with sodium azide .

Applications in Research

RTP1 antibodies are pivotal in:

• Western Blotting (WB): Detecting RTP1 expression in tissue lysates or cell lines .

• Immunohistochemistry (IHC): Localizing RTP1 in olfactory epithelium or brain sections .

• Functional Studies: Investigating RTP1’s role in olfactory receptor trafficking and GPCR regulation .

Quality Control and Validation

• Specificity: Confirmed via knockout cell lines or peptide blocking assays .

• Buffers: Contain sodium azide (0.02–0.09%), requiring careful handling .