RWDD1 Human

RWD Domain Containing 1 Human Recombinant
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Cat. No.
BT19366
Source
Escherichia Coli.
Synonyms
RWD domain-containing protein 1, DRG family-regulatory protein 2, RWDD1, DFRP2, CGI-24, PTD013.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Functional Roles

Primary Function
RWDD1 stabilizes DRG2 (Developmentally-regulated GTP-binding protein 2) by preventing its degradation, ensuring proper GTP hydrolysis and cellular signaling .

Biological Processes

  • Androgen Receptor Signaling: Modulates androgen receptor activity and testosterone responses .

  • Cellular Stress Response: Involved in oxidative stress mitigation and aging .

  • Translation Regulation: Interacts with proteins like DENR and GCN1 to influence mRNA translation .

Protein Interactions

RWDD1 forms functional partnerships with multiple proteins, as evidenced by interaction networks :

PartnerFunctionInteraction Score
DRG2GTP-binding protein; involved in RNA binding and translation when hydroxylated 0.999
DRG1Microtubule polymerization; GTPase activity stimulated by ZC3H15 0.982
ZC3H15Protects DRG1; enhances GTPase activity via potassium ion affinity 0.917
DENRPromotes ribosomal translation initiation; interacts with MCTS1 0.834
GCN1Activates GCN2 kinase in response to amino acid starvation 0.767

Research Applications

Experimental Tools

  • Recombinant Protein: Available as E. coli-expressed, His-tagged protein (30.3 kDa) for in vitro studies .

  • Antibodies: Polyclonal antibodies validated for Western blot (WB), immunohistochemistry (IHC), and immunoprecipitation (IP) .

Cancer Research
RWDD1 expression is observed in multiple cancers, including breast, prostate, and lung, with potential implications in oncogenic pathways . Survival correlations remain under investigation.

Chemical and Environmental Interactions

RWDD1 expression is modulated by diverse chemical agents, as shown in preclinical studies :

ChemicalEffect on RWDD1Model Organism
4,4'-DiaminodiphenylmethaneIncreases mRNA expression Rat
Bisphenol ADecreases mRNA expression Mouse
Valproic AcidIncreases mRNA expression (synergistic with hydralazine) Rat
Aflatoxin B1Decreases gene methylation Human
Acrolein + PollutantsDecreases mRNA expression in air pollution models Human

Gene and Expression Data

Gene Information

ParameterDetail
Gene ID51389 (Human)
mRNA VariantsMultiple isoforms, including transcript variants from BC015802
Expression PatternsUbiquitous, with elevated levels in calcaneal tendon and immune tissues

Protein Stability

  • Storage: Stable at 4°C (2–4 weeks) or -20°C (long-term). Avoid freeze-thaw cycles .

  • Purity: >85% via SDS-PAGE .

Future Research Directions

  • Therapeutic Potential: Exploring RWDD1’s role in androgen receptor-driven cancers.

  • Environmental Toxicology: Mechanistic studies on chemical-induced epigenetic changes.

  • Structural Biology: Resolving RWD domain interactions with DRG proteins.

RWDD1’s multifaceted roles in cellular homeostasis and disease make it a compelling target for advanced biochemical and clinical research.

Product Specs

Introduction
RWD Domain Containing 1 (RWDD1), a member of the RWDD1/GIR2 family, safeguards DRG2 (developmentally regulated GTP binding protein 2) from degradation by proteolysis. The RWD domain is characterized by three signature motifs found in proteins: RING finger, WD-repeats, and a motif resembling yeast DEAD (DEXD). Existing research suggests that the RWD domain might be essential for interactions between proteins.
Description
Recombinantly produced in E. coli, RWDD1 Human Recombinant is a single, non-glycosylated polypeptide chain consisting of 266 amino acids (specifically, amino acids 1 to 243) with a molecular weight of 30.3 kDa. Note that its size on SDS-PAGE will appear larger. This RWDD1 protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution that has been filtered.
Formulation
The RWDD1 protein solution is provided at a concentration of 0.5 mg/ml and contains 20mM Tris-HCl buffer at a pH of 8.0, 0.1M NaCl, 10% glycerol, and 2mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For longer storage, it should be frozen and stored at -20°C. The addition of a carrier protein (either 0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing of the product.
Purity
SDS-PAGE analysis has determined the purity to be greater than 85.0%.
Synonyms
RWD domain-containing protein 1, DRG family-regulatory protein 2, RWDD1, DFRP2, CGI-24, PTD013.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTDYGEE QRNELEALES IYPDSFTVLS ENPPSFTITV TSEAGENDET VQTTLKFTYS EKYPDEAPLY EIFSQENLED NDVSDILKLL ALQAEENLGM VMIFTLVTAV QEKLNEIVDQ IKTRREEEKK QKEKEAEEAE KQLFHGTPVT IENFLNWKAK FDAELLEIKK KRMKEEEQAG KNKLSGKQLF ETDHNLDTSD IQFLEDAGNN VEVDESLFQE MDDLELEDDE DDPDYNPADP ESDSAD.

Product Science Overview

Gene and Protein Structure

The RWDD1 gene is located on chromosome 6q22.1 . The protein encoded by this gene consists of 243 amino acids and has an N-terminal RWD domain . The RWD domain is thought to be involved in protein-protein interactions, although its exact function is not fully understood .

Expression and Localization

RWDD1 is expressed in various tissues, with high expression levels observed in the thymus of young mice . However, its expression decreases significantly in aging mice and in those subjected to oxidative stress . In humans, RWDD1 is ubiquitously expressed, with notable enrichment in the cytosol of urethral epithelium .

Function and Interactions

RWDD1 functions as a transcription factor that regulates the expression of target genes . It has been shown to interact with the androgen receptor (AR) and enhance AR-dependent transactivation . Unlike many AR coregulators, RWDD1’s enhancement of AR-dependent transactivation is independent of the formation of the activation function-2 (AF2) hydrophobic protein-binding pocket in AR . Additionally, RWDD1 also activates glucocorticoid receptor (GR)-dependent transactivation .

Recombinant Human RWDD1

Recombinant human RWDD1 protein is produced using conventional chromatography techniques and is often tagged with a His-tag at the N-terminus for purification purposes . This recombinant protein is used in various research applications to study the function and interactions of RWDD1.

Clinical Relevance

While the exact clinical implications of RWDD1 are still being explored, its role in transcription regulation and interaction with key receptors like AR and GR suggests potential involvement in various physiological and pathological processes .

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