RZ1C Antibody

Functional Roles in Plant Development

Loss of RZ-1B and RZ-1C leads to developmental defects:

RNA Splicing Regulation

• RZ-1C binds pre-mRNA and facilitates splicing by recruiting SR proteins .

• RNA-seq of rz-1b rz-1c mutants revealed widespread splicing defects in >1,000 genes .

Chromatin Interaction

• RZ-1C promotes H3K27me3 deposition at Polycomb Repressive Complex 2 (PRC2) targets, such as FLC, to repress transcription .

• CLNIP-MS identified 18 SR proteins as interaction partners, linking RZ-1C to chromatin remodeling .

Genetic Interactions

Global Gene Expression Impact

• Over 3,000 genes exhibit altered expression in rz-1b rz-1c mutants, including developmental regulators .

• H3K27me3 levels at PRC2 targets are reduced by 30–50% in mutants during early germination .

Experimental Tools and Antibody Applications

While the term "RZ1C antibody" is not explicitly defined in the literature, studies use GFP-RZ-1C fusions for localization and interaction assays, implying the use of anti-GFP antibodies for detection . Key methodologies include:

• Yeast two-hybrid screens: Identified SR proteins as interaction partners .

• Chromatin immunoprecipitation (ChIP): Demonstrated RZ-1C’s role in H3K27me3 dynamics .

Implications and Future Directions

RZ-1C bridges RNA metabolism and chromatin regulation, offering insights into:

• Coordination between splicing and epigenetic silencing .

• ABA signaling modulation during stress responses .

• Evolutionary conservation of SR protein networks in eukaryotes .