S100A8 Human, His

S100 Calcium Binding Protein A8 Human Recombinant, His Tag
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Cat. No.
BT587
Source
Escherichia Coli.
Synonyms
Calgranulin A, MRP8, CAGA, CGLA, CFAG, Protein S100-A8, S100 calcium-binding protein A8, Migration inhibitory factor-related protein 8, MRP-8, p8, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Calprotectin L1L subunit, Urinary stone protein band A, S100A8, MIF, NIF, L1Ag, CP-10, MA387, 60B8AG.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Inflammation and Immune Regulation

  • Chemotaxis and Migration: S100A8 promotes neutrophil chemotaxis and phagocyte migration via tubulin polymerization .

  • Cytokine Modulation: Upregulates NF-κB-dependent genes, IL-8, and ICAM-1, amplifying inflammatory responses .

  • Oxidative Metabolism: Enhances NADPH-oxidase activity by facilitating arachidonic acid transfer .

Cancer Progression

  • Metastasis: Recombinant S100A8/A9 promotes cancer cell migration and invasion via Akt/p38 MAPK signaling .

  • Biomarker Potential: Elevated S100A8 levels correlate with poor survival in breast, lung, and pancreatic cancers .

Cardiovascular Disease

  • Heart Failure Prediction: Circulating S100A8/A9 predicts post-acute myocardial infarction (AMI) heart failure with a hazard ratio of 1.92 per standard deviation .

Production and Purification

Recombinant S100A8 Human, His is produced in E. coli, purified using nickel affinity chromatography, and validated via SDS-PAGE . Key steps include:

  1. Cloning: S100A8 cDNA inserted into pET28 vector .

  2. Expression: Induced with IPTG at 16°C to optimize folding .

  3. Tag Removal: His-tag cleaved by thrombin, followed by endotoxin removal .

ParameterSpecification
Expression VectorpET28
Tag RemovalThrombin cleavage
Endotoxin Levels<1 pg/μg protein

Therapeutic Targets

  • Inflammatory Diseases: S100A8/A9 inhibitors show promise in rheumatoid arthritis and sepsis .

  • Cancer Therapy: Targeting S100A8/A9 reduces metastasis and chemoresistance in preclinical models .

Diagnostic Use

  • Biomarker: S100A8/A9 serum levels are elevated in colorectal carcinoma and hepatocellular carcinoma .

Key Research Findings

Study FocusKey OutcomeCitation
Post-AMI Heart FailureS100A8/A9 predicts HF with HR = 2.03 (FG model)
Cancer MetastasisS100A8/A9 activates Akt/p38 MAPK in esophageal SCC
Neutrophil ActivationS100A8 induces chemotaxis and adhesion

Product Specs

Introduction
S100A8, a member of the S100 protein family, possesses two EF-hand calcium-binding motifs. Found in the cytoplasm and/or nucleus of various cells, S100 proteins regulate cellular processes like cell cycle progression and differentiation. S100A8 functions as a casein kinase inhibitor and a cytokine, with its altered expression linked to cystic fibrosis. This calcium-binding protein exhibits antimicrobial properties against bacteria and fungi, playing a crucial role in resisting pathogenic bacterial invasion. S100A8 upregulates NF-κB-controlled gene transcription and contributes to endotoxic shock development in response to bacterial lipopolysaccharide. It facilitates tubulin polymerization, promoting phagocyte migration and granulocyte infiltration at wound sites. As a pro-inflammatory mediator in acute and chronic inflammation, S100A8 upregulates IL-8 release and ICAM1 cell-surface expression.
Description
Recombinant Human S100A8, expressed in E. coli, has a molecular weight of 12.08 kDa. It comprises 103 amino acids of the human S100A8 protein with a 10-amino acid His tag fused at the N-terminus.
Formulation
S100A8 is supplied lyophilized at a concentration of 0.5 mg/ml in a buffer of 20mM Tris and 100mM NaCl, pH 7.5. The product has been sterile filtered through a 0.4 μm filter.
Solubility
To prepare a working stock solution, add deionized water to the lyophilized pellet to achieve a concentration of approximately 0.5 mg/ml. Ensure complete dissolution of the pellet. This product is not sterile. Prior to cell culture use, filter the solution through an appropriate sterile filter.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated freeze-thaw cycles. The reconstituted protein demonstrates stability at 4°C for a limited period and remains stable for up to two weeks at this temperature.
Synonyms
Calgranulin A, MRP8, CAGA, CGLA, CFAG, Protein S100-A8, S100 calcium-binding protein A8, Migration inhibitory factor-related protein 8, MRP-8, p8, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Calprotectin L1L subunit, Urinary stone protein band A, S100A8, MIF, NIF, L1Ag, CP-10, MA387, 60B8AG.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS MLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG ADVWFKELDI NTDGAVNFQEMLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG ADVWFKELDI NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKE.

Product Science Overview

Introduction

S100 Calcium Binding Protein A8, also known as S100A8 or calgranulin A, is a member of the S100 family of proteins. These proteins are characterized by their ability to bind calcium through EF-hand motifs, which are helix-loop-helix structural domains . S100A8 is predominantly found in the cytoplasm and/or nucleus of a wide range of cells and plays a crucial role in regulating various cellular processes, including cell cycle progression and differentiation .

Structure and Function

S100A8 is a calcium- and zinc-binding protein that forms a heterodimer with S100A9, known as calprotectin . This heterodimer has a wide range of intra- and extracellular functions, particularly in the regulation of inflammatory processes and immune responses . S100A8 can induce neutrophil chemotaxis and adhesion, making it a significant player in the body’s defense mechanisms .

Genetic Information

The S100A8 gene is located on chromosome 1q21 and is part of a cluster of at least 13 S100 genes . Multiple transcript variants encoding different isoforms of S100A8 have been identified, indicating the complexity and versatility of this protein .

Clinical Significance

Altered expression of S100A8 is associated with various diseases, including cystic fibrosis and inflammatory conditions . The protein’s role in inflammation and immune response makes it a potential biomarker for these diseases and a target for therapeutic interventions .

Recombinant S100A8

The human recombinant form of S100A8, tagged with a His (histidine) tag, is produced using recombinant DNA technology. This involves inserting the gene encoding S100A8 into an expression vector, which is then introduced into a host cell (commonly E. coli) to produce the protein. The His tag facilitates the purification of the recombinant protein through affinity chromatography, allowing for the isolation of high-purity S100A8 for research and clinical applications.

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