SAUR50 Antibody
Description
SAUR50: Molecular Context and Functional Insights
SAUR50 belongs to the Small Auxin-Up RNA (SAUR) family, encoding short-lived proteins that mediate auxin signaling in plants. Key findings include:
SAUR50 overexpression correlates with:
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Cotyledon opening during de-etiolation.
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Hypocotyl elongation via cell wall loosening and apoplastic acidification.
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Interaction with PP2C-D phosphatases, which modulate H⁺-ATPase activity and auxin-induced growth.
Antibody Development: Methodological Parallels
Though no direct evidence of SAUR50-specific antibodies exists, standard antibody production strategies for plant proteins (e.g., TCP4, PP2C-D1) provide a framework:
Research Gaps and Future Directions
The absence of SAUR50-specific antibody studies highlights critical gaps:
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Technical Challenges: SAUR proteins are small (~10–15 kDa), making antibody generation difficult due to limited epitopes.
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Functional Redundancy: SAUR50 may share epitopes with other SAURs (e.g., SAUR16, SAUR77), complicating specificity.
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Experimental Priorities: Focus on downstream targets (e.g., PP2C-D1, AHA H⁺-ATPases) may have diverted attention from SAUR50 itself.
SAUR50’s Role in Auxin-Driven Growth: Key Mechanisms
SAUR50’s mechanism aligns with the acid growth theory:
| Process | SAUR50’s Role | Auxin Pathway Link |
|---|---|---|
| Apoplastic Acidification | Indirectly enhances H⁺-ATPase activity by inhibiting PP2C-D1 phosphatases. | ABP1-TMK signaling cascade |
| Cell Wall Remodeling | Promotes expansion via cell wall loosening and turgor pressure maintenance. | GH3/ Aux/IAA gene regulation |
| Light Signaling | Acts downstream of TCP4 to enable rapid cotyledon opening during photomorphogenesis. | PIF-TCP antagonism |
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
