SBT3.3 Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- SBT3.3 has been identified as a key player in epigenetic control of plant immunity. Research indicates that SBT3.3 is upregulated and priming is activated when epigenetic control is disrupted. This suggests that SBT3.3 serves as a novel regulator of primed immunity. PMID: 23818851
Q&A
FAQs for SBT3.3 Antibody in Academic Research
What experimental approaches validate SBT3.3 antibody specificity in Arabidopsis?
To confirm antibody specificity:
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Western blot with knockout mutants: Compare protein extracts from wild-type and SBT3.3 knockout lines. Absence of a band in knockouts confirms specificity .
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Peptide competition assays: Pre-incubate the antibody with the antigenic peptide used for immunization. Loss of signal indicates specificity .
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Immunolocalization consistency: Cross-validate with transgenic lines expressing fluorescently tagged SBT3.3 (e.g., GFP fusions) .
Which pathogens are used to study SBT3.3-mediated resistance?
SBT3.3 is tested against pathogens with distinct lifestyles:
How is SBT3.3 expression modulated during immune responses?
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Hormonal treatments: Expose plants to salicylic acid (SA) or methyl jasmonate (MeJA) and quantify SBT3.3 transcript levels via qRT-PCR .
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Pathogen inoculation: Time-course experiments post-inoculation (e.g., 0, 24, 48 h.p.i.) with immunoblotting to track protein accumulation .
How does SBT3.3 coordinate SA-JA cross-talk during pathogen challenges?
SBT3.3 enhances SA signaling while suppressing JA pathways:
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Method: Use NahG (SA-deficient) and coi1 (JA-insensitive) mutants to assess dependency.
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Data contradiction: Some studies report JA suppression via SBT3.3 , while others note context-dependent synergies . Resolve by profiling PDF1.2 (JA marker) and PR1 (SA marker) in overexpression lines .
What structural features of SBT3.3 are critical for its protease activity?
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Computational modeling: Use tools like RosettaAntibodyDesign (RAbD) to predict active-site residues .
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Site-directed mutagenesis: Mutate catalytic triad residues (e.g., Asp-His-Ser) and test protease activity via caseinolytic assays .
How to optimize co-immunoprecipitation (Co-IP) assays for SBT3.3 interaction studies?
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Controls: Include IgG isotype controls and sbt3.3 knockout extracts to exclude nonspecific binding .
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Buffer optimization: Use high-stringency buffers (e.g., 150–300 mM NaCl) to reduce background noise .
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Crosslinkers: Apply formaldehyde for transient interactions (e.g., pathogen effector binding) .
