At4g32640 Antibody

Protein Trafficking Studies

The antibody identifies SEC24C in COPII-mediated vesicle formation, crucial for understanding:

• Cargo selectivity during ER export .

• Mutant phenotypes (e.g., sec24c-1) showing disrupted heavy metal tolerance (Cd(II), As(III), As(V)) .

Stress Response Mechanisms

Studies using this antibody revealed:

• SEC24C’s role in mitigating cadmium and arsenic toxicity .

• Phenotypic similarities between sec24c-1 and cas2 mutants under metal stress :

Functional Validation

• Genetic Evidence: sec24c-1 T-DNA insertion mutants exhibit identical sensitivity profiles to cas2 mutants, confirming SEC24C as the causal locus for cadmium/arsenic responses .

• Protein Interaction Mapping: Used to immunoprecipitate SEC24C-containing complexes, aiding in identifying interactors like SEC23 and cargo receptors .

Technical Advantages

• High Specificity: Recognizes endogenous SEC24C without cross-reactivity to other SEC24 isoforms .

• Versatility: Compatible with multiple plant tissue types (roots, leaves) and subcellular fractionation protocols .