SCAMP1 Antibody, HRP conjugated

Key Features

2.1. ELISA and Detection Sensitivity

The HRP-conjugated SCAMP1 antibody is optimized for ELISA-based quantification. In pancreatic adenocarcinoma (PAAD) studies, SCAMP1 overexpression correlates with poor prognosis , making this antibody critical for biomarker validation.

2.2. Western Blot and Immunoprecipitation

• Western Blot: Detects a ~31–37 kDa band in lysates from HeLa, rat brain, and mouse tissues .

• Immunoprecipitation: Used to isolate SCAMP1 complexes, such as those involving SCAMP3 in CHO cells .

2.3. Role in Cancer Research

SCAMP1 is implicated in tumor progression:

• Pancreatic Cancer: Elevated SCAMP1 expression associates with advanced TNM staging and neural invasion .

• Glioma: SCAMP1 knockdown reduces proliferation and migration, suggesting oncogenic roles .

• Hepatitis B Virus (HBV): SCAMP1 inhibits HBV replication by modulating endocytic pathways .

3.1. Cross-Reactivity and Specificity

The antibody’s epitope (e.g., residues 1–17 in human SCAMP1 ) ensures minimal cross-reactivity with other SCAMP family members (SCAMP2–5) .

4.1. Therapeutic Potential

SCAMP1’s role in modulating viral replication (e.g., HBV ) and cancer progression highlights its potential as a therapeutic target. HRP-conjugated antibodies may aid in developing SCAMP1-based diagnostics or drug delivery systems.

4.2. Limitations

• Tissue Specificity: SCAMP1 is ubiquitously expressed, requiring careful validation in specific cellular contexts.

• Conjugation Stability: HRP activity may degrade under non-optimal storage conditions .