slc25a24 Antibody

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Cat. No.
BT1706740
Synonyms
slc25a24; scamc1; si:ch211-63o20.9; zgc:92470; Calcium-binding mitochondrial carrier protein SCaMC-1; Small calcium-binding mitochondrial carrier protein 1; Solute carrier family 25 member 24
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Description

Definition and Function of SLC25A24 Antibody

The SLC25A24 antibody is a laboratory reagent designed to detect and quantify the SLC25A24 protein, a mitochondrial ATP-Mg/phosphate carrier critical for energy homeostasis . It facilitates studies on the protein’s role in metabolic pathways, calcium buffering, and disease mechanisms .

Applications and Validation Data

SLC25A24 antibodies are validated for diverse experimental workflows:

ApplicationDetailsExample Antibodies
Western Blot (WB)Detects ~48–53 kDa bands in HEK-293 cells, mouse heart, and human placenta tissues .Proteintech 14669-1-AP (1:1000–1:6000 dilution) , Abcam ab221120 (1:100 dilution) .
Immunohistochemistry (IHC)Localizes SLC25A24 in paraffin-embedded tissues (e.g., human duodenum, mouse testis) .DSHB PCRP-SLC25A24-3G2 (mouse monoclonal) , Proteintech 14669-1-AP .
Immunofluorescence (IF)Confirms mitochondrial localization in A431 cells and colon cancer specimens .Abcam ab221120 , Proteintech 14669-1-AP .
ELISAQuantifies recombinant SLC25A24 protein using epitope-specific binding .Antibodies-Online ABIN526192 (mouse polyclonal) .

Role in Metabolic Disorders

  • Knockout Mice Studies:

    • Slc25a24-knockout mice exhibit resistance to high-fat diet-induced obesity, reduced adipocyte differentiation markers (Pparγ, C/ebpα), and lower triglyceride deposition in the liver .

    • SLC25A24 depletion correlates with impaired mitochondrial cristae structure and energy metabolism .

Prognostic Value in Cancer

StudyKey InsightCitation
Colon cancer prognosisSLC25A24 expression inversely linked to tumor immune evasion and metastasis.
Mitochondrial dysfunctionMutations cause swollen mitochondria and abnormal cristae in fibroblasts.

Antibody Characteristics

ParameterDetails
Host SpeciesRabbit (polyclonal) , Mouse (monoclonal) .
ImmunogenRecombinant SLC25A24 protein fragments (e.g., aa 1-477) .
ReactivityHuman, mouse, rat .
Storage-20°C (PBS with 0.02% sodium azide) or -80°C (PBS-only buffer) .

Cross-Reactivity Notes

  • Proteintech’s antibody shows 100% homology with mouse and rat SLC25A24 .

  • Abcam’s ab221120 reacts exclusively with human samples .

Clinical and Mechanistic Insights

  • Calcium Buffering: SLC25A24 protects against oxidative stress by modulating mitochondrial calcium-phosphate precipitates .

  • Adenyl Nucleotide Transport: Acts as a broad-specificity antiporter for ATP, ADP, and AMP, influencing metabolic pathways like glycolysis .

Limitations and Future Directions

  • Current antibodies lack validation in in vivo imaging or flow cytometry .

  • Further studies are needed to clarify SLC25A24’s role in glucose tolerance and insulin resistance .

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
slc25a24; scamc1; si:ch211-63o20.9; zgc:92470; Calcium-binding mitochondrial carrier protein SCaMC-1; Small calcium-binding mitochondrial carrier protein 1; Solute carrier family 25 member 24
Target Names
slc25a24
Uniprot No.
Q66L49

Target Background

Function
SLC25A24 is a calcium-dependent mitochondrial solute carrier. It facilitates the reversible, electroneutral exchange of Mg-ATP or Mg-ADP against phosphate ions, mediating the net uptake or efflux of adenine nucleotides across the mitochondrial inner membrane. Nucleotide transport is inactive at low cytosolic calcium concentrations and is activated by an increase in cytosolic calcium levels. SLC25A24 may play a role in protecting cells against oxidative stress-induced cell death. This is likely achieved by promoting the formation of calcium-phosphate precipitates in the mitochondrial matrix, thus buffering calcium levels within the mitochondrial matrix.
Database Links

KEGG: dre:447867

STRING: 7955.ENSDARP00000033055

UniGene: Dr.82440

Protein Families
Mitochondrial carrier (TC 2.A.29) family
Subcellular Location
Mitochondrion inner membrane; Multi-pass membrane protein.

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