SCPL3 Antibody

SPPL3 Antibody: Role in Immune Regulation

SPPL3 is an aspartyl protease that regulates glycosphingolipid (GSL) metabolism by controlling the enzyme B3GNT5. Its activity impacts HLA-I-mediated immune responses, as demonstrated in genome-wide screens .

Key Research Findings:

• Mechanism of Action:
  SPPL3 degrades B3GNT5, limiting the synthesis of neolacto-series GSLs (nsGSLs). Elevated nsGSLs in SPPL3-deficient cells sterically hinder HLA-I interactions with immune receptors like CD8+ T cells .
  $\text{SPPL3} \rightarrow \text{B3GNT5 inhibition} \rightarrow \text{nsGSLs} \downarrow \rightarrow \text{HLA-I accessibility} \uparrow$

• Functional Impact:

  • Reduced SPPL3 expression correlates with impaired T cell activation and tumor immune evasion .

  • In gliomas, dysregulated SPPL3-B3GNT5 pathways are linked to poorer patient survival .

SCP3/SYCP3 Antibody: Applications in Meiosis Research

SCP3 (SYCP3) is a structural protein critical for synaptonemal complex formation during meiosis. Antibodies against SCP3 are widely used in reproductive biology and cancer research .

Antibody Characteristics:

• Host/Isotype: Mouse IgG1 (common across clones like 6F9C5 and 66409-1-Ig) .

• Applications:

  • Western Blot: Detects ~30 kDa band in human testis and cancer cell lines .

  • Immunohistochemistry (IHC): Localizes to spermatogonia and spermatocytes in testis .

  • Immunofluorescence (IF): Visualizes chromosome synapsis defects in oocytes .

Clinical Relevance:

• Mutations in SYCP3 are associated with male infertility (azoospermia) and recurrent pregnancy loss .

• SCP3 overexpression in cervical and kidney cancers suggests potential diagnostic utility .

Comparative Analysis of SPPL3 vs. SCP3 Antibodies

Future Directions

• SPPL3: Explore nsGSL inhibition in combination therapies for glioma .

• SCP3: Investigate its role in chemotherapy-induced meiotic defects .