At3g59480 Antibody
Description
Applications in Research
The At3g59480 antibody is primarily utilized for:
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Western Blotting: Detecting endogenous levels of the At3g59480 protein in Arabidopsis tissue lysates.
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Immunohistochemistry: Localizing the protein in plant tissue sections.
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Enzyme-Linked Immunosorbent Assay (ELISA): Quantifying protein expression under experimental conditions .
Research Findings and Utility
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Metabolic Studies: In tomato, the fructokinase-2 homolog (SlFRK2, UniProt: Q9M1B9 ortholog) shows increased expression under gamma radiation, suggesting a role in managing oxidative stress . This implies that At3g59480 in Arabidopsis may have analogous functions in carbohydrate metabolism during stress.
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Antibody Validation: While the At3g59480 antibody’s specificity is inferred from its design, standard validation methods (e.g., peptide blocking assays or knockout line testing) are recommended to confirm signal reliability .
Comparative Analysis
| Feature | At3g59480 Antibody | General Plant Antibody Trends |
|---|---|---|
| Target Specificity | High (designed for Arabidopsis protein) | Variable (often cross-reactive) |
| Applications | Metabolic, stress-response studies | Broad (development, pathology) |
| Commercial Availability | Specialized (Cusabio) | Wide (multiple vendors) |
Limitations and Future Directions
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Functional Data Gap: Direct evidence linking At3g59480 to specific pathways in Arabidopsis remains sparse.
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Technical Optimization: Antibody performance may vary depending on sample preparation and experimental conditions.
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
