SDC Antibody

Table 1: Representative SDC Antibodies and Their Properties

*MW: Molecular weight. Sources: .

Immunohistochemistry (IHC) and Flow Cytometry

• IHC Protocol: Heat-mediated antigen retrieval (Tris/EDTA buffer, pH 9.0) is critical for optimal detection in paraffin-embedded tissues .

• Flow Cytometry: SDC1 antibodies like ab128936 and ARG66252 exhibit strong signals in fixed/permeabilized cells (e.g., HeLa, Raji) .

Western Blotting (WB)

• SDC1 migrates as multiple bands (35–250 kDa) due to post-translational modifications (e.g., glycosylation) .

• Example: ab128936 detects a 100 kDa band in Ramos cells and a 35 kDa band in Raji cells .

Functional Studies

• Immune Regulation: SDC1 deficiency reduces T cell proliferation and IL-17 production in dendritic cell (DC)–T cell co-cultures .

• Neurodegeneration: Syndecans mediate α-synuclein and tau fibril uptake in neuronal cells, implicating SDC1 in prion-like protein spread .

Cancer and Diagnostics

• Biomarker Potential: SDC1 is overexpressed in multiple myeloma (as CD138) and breast cancer, making it a target for antibody-based therapies .

• Therapeutic Target: Soluble SDC1 modulates CXCL1 activity, influencing neutrophil recruitment in tumors .

Table 2: Clinical Associations of SDC1

Challenges and Considerations

• Antibody Specificity: Cross-reactivity with glycosylated SDC1 variants requires validation using knockout controls .

• Species Limitations: Most antibodies target human SDC1; reactivity in non-human models (e.g., mouse) is limited .