P85 Antibody
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In Stock 
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
P85 antibody; At4g35985 antibody; T19K4.110 antibody; Senescence/dehydration-associated protein At4g35985 antibody; chloroplastic antibody
Target Names
P85
Uniprot No.
Target Background
Database Links
Subcellular Location
Plastid, chloroplast.
Tissue Specificity
Expressed in leaves (especially in midribs and trichomes), apical meristemic regions, stems, roots and flowers.
Q&A
The following FAQs address key methodological considerations for researchers working with p85 antibodies in academic settings, synthesized from peer-reviewed studies and technical documentation. Questions are categorized by complexity, with emphasis on experimental design and data interpretation.
Advanced Research Questions
How to resolve contradictory p85-PTEN interaction data across cell lines?
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Consider phosphorylation status: Unphosphorylated PTEN preferentially binds p85α/p110β complexes .
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Validate using:
What strategies differentiate p85α/p85β functional roles in PI3K signaling?
| Approach | Key Readout | Example Study |
|---|---|---|
| siRNA knockdown | AKT phosphorylation kinetics | MM cell lines with FGFR3 mutations |
| Isoform-specific IP | p110 subunit association profiles | MEFs from p85α/β KO mice |
How to design cross-species experiments given p85 antibody reactivity?
Methodological Notes from Key Studies
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Co-IP optimization: Reduced p85 detection in heterozygous MEFs requires increased lysate input (≥500 μg) .
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Flow cytometry: Fixation with 4% PFA + 0.1% saponin improves intracellular epitope accessibility .
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Phosphorylation studies: Anti-p85 (pTyr467/p55pTyr199) antibodies require ligand stimulation timing optimization .
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