scgn Antibody

SCGN Antibody Overview

The SCGN antibody (CAB12897) is a rabbit polyclonal antibody designed for detecting human, mouse, and rat Secretagogin. It is widely used in Western blotting (WB), immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA .

Biological Role of SCGN

SCGN is a hexa–EF-hand calcium sensor highly expressed in pancreatic β-cells, neurons, and enteroendocrine cells. Key functions include:

• Insulin Secretion: Facilitates glucose-stimulated insulin release in β-cells .

• Neuroprotection: Mitigates oxidative stress and excitotoxicity in neurons .

• Exocytosis Regulation: Binds SNAP-25, a SNARE protein, to modulate vesicle fusion and hormone secretion .

SCGN deficiency is linked to diabetes, neurodegenerative disorders (e.g., Alzheimer’s), and inflammatory bowel disease .

Pancreatic β-Cell Survival

• Lipotoxicity Effects: Downregulation of SCGN in MIN6 β-cells under lipotoxic conditions (e.g., ox-LDL exposure) inhibits proliferation and induces cell death .

• Mechanistic Insight: SCGN knockdown mimics ox-LDL cytotoxicity, underscoring its role in β-cell survival .

SNARE Complex Regulation

• SNAP-25 Interaction: SCGN binds SNAP-25 in a Ca²⁺-dependent manner, inhibiting SNARE-mediated vesicle fusion. Structural studies reveal SCGN’s EF-hands 5–6 as critical binding sites .

• Cellular Localization: SCGN promotes plasma membrane localization of SNAP-25 but not Syntaxin-1a. Loss of SCGN disrupts SNAP-25 trafficking, impairing exocytosis .

Applications in Disease Research

• Diabetes: SCGN expression correlates with β-cell function; its downregulation exacerbates hyperglycemia in murine models .

• Neurodegeneration: Altered SCGN levels in brain tissues are observed in Alzheimer’s and Parkinson’s disease .

• Cancer: SCGN’s role in cell proliferation links it to tumorigenesis, though mechanisms remain under investigation .

Technical Considerations

• Antibody Validation: CAB12897 detects SCGN in mouse brain, eye, and rat pancreas lysates .

• Cross-Reactivity: No reported cross-reactivity with other EF-hand proteins (e.g., calbindin D-28K) .

• Storage: Stable in PBS with 0.01% thimerosal and 50% glycerol at -20°C .

Future Directions

Current research focuses on:

1. SCGN’s role in hormone secretion beyond insulin (e.g., corticotropin-releasing hormone) .

2. Therapeutic targeting of SCGN-SNAP-25 interactions for metabolic and neurological disorders .

3. Development of SCGN-based biomarkers for early disease detection .