SEN1 Antibody
Description
Biological Role of SEN1
SEN1 is a superfamily I helicase with key functions in:
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Transcription termination: Resolving RNA polymerase II (RNAPII) and III (RNAPIII) transcription complexes
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R-loop prevention: Resolving RNA:DNA hybrids to maintain genome stability
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Replication fork progression: Interacting with replisome components (e.g., Ctf4, Mrc1) during DNA replication
Table 1: Key Functional Domains of SEN1
Disease Relevance
SEN1 homologs are linked to human diseases:
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Senataxin (SETX): Mutations cause ALS4 and ataxia with oculomotor apraxia type 2 .
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Mechanism: Defective resolution of R-loops and transcription-replication conflicts .
Cell-Cycle Regulation
SEN1 levels fluctuate during the cell cycle:
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Degradation in G1: Mediated by ubiquitin-proteasome system .
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Stabilization in S/G2: Critical for replication fork progression .
Experimental Tools for SEN1 Study
While no SEN1-specific antibody is described in the sources, methodologies include:
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Chromatin immunoprecipitation (ChIP-seq): Maps SEN1 binding to RNAPIII-transcribed genes (tRNA, 5S rRNA) .
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Temperature-sensitive mutants: sen1-1 (G1747D) used to study transcription termination defects .
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CRAC (UV crosslinking and analysis of cDNA): Identifies SEN1-RNAPII interaction sites .
Research Gaps and Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- The helicase domain (HD) is sufficient to dissociate the elongation complex (EC) in vitro. Both full-length Sen1 and its HD can translocate along single-stranded RNA and DNA in the 5 to 3 direction. PMID: 28180347
- Research has identified new targets and functions of Sen1-dependent termination, including a role in the repression of meiotic genes in vegetative cells. These findings, combined with other recent whole-genome studies on the termination of non-coding RNAs, provide promising avenues for further investigation. PMID: 28665995
- Studies have shown that subdomain 1C (the "prong") is crucial for 5'-3' unwinding of RNA and for Sen1-mediated transcription termination in vitro. PMID: 28408439
- Mutations in the DNA/RNA helicase Sen1 can modulate the protein-serine-threonine kinase TOR pathway and autophagy. PMID: 27718307
- Data suggests that mutants of the RNA processing machinery (RNT1) and RNA/DNA helicase SEN1 mutants that cannot interact with Rnt1p exhibit a flocculation phenotype. PMID: 26364722
- Findings are consistent with a model where Sen1 promotes transcription termination by resolving R-loops. PMID: 26198638
- Research provides evidence that topological stress contributes to the recruitment and/or stabilization of condensin at Pol III-transcribed genes in the absence of Swd2.2 and Sen1. PMID: 25392932
- Multiple domains of Sen1 are involved in transcription termination. PMID: 25116135
- Sen1p recognizes the nascent RNA and hydrolyzes ATP to dissociate the elongation complex. Sen1p-dependent transcription termination is highly specific and does not require the C-terminal domain of RNAPII. PMID: 23748379
- Studies have shown that the Sen1/Senataxin DNA/RNA helicase associates with forks, promoting their progression across RNA polymerase II (RNAPII)-transcribed genes. PMID: 23141540
- Analysis of a homogeneous set of several hundred artificial, Nrd1-Nab3-Sen1 complex-dependent terminators revealed novel and extended sequence determinants for transcription termination, as well as supermotifs critical for termination. PMID: 23032188
- The transcriptome-wide distribution of termination factors Nrd1, Nab3, and Sen1 has been reported. PMID: 22028667
- Research shows that Nrd1, Nab3, and Sen1 bind to a number of noncoding RNAs in an unexpected manner. PMID: 21954178
- Data suggests that Set1 promotes proper early termination by the Nrd1-Nab3-Sen1 complex by affecting the kinetics of Pol II transcription in early elongation. PMID: 21709022
- Findings reveal a regulatory mechanism where Mpk1 overcomes transcriptional attenuation by blocking recruitment of the Sen1-Nrd1-Nab3 termination complex to the elongating polymerase. PMID: 21376235
- Sen1p-Rpb1p and Sen1p-Rnt1p interactions occur independently of each other and serve genetically separable purposes in targeting Sen1p to function in two temporally overlapping steps in gene expression. PMID: 19884310
- The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. PMID: 18660819
KEGG: sce:YLR430W
STRING: 4932.YLR430W
