Serpine1 Antibody
Description
Definition and Biological Context
SERPINE1 Antibody specifically binds to plasminogen activator inhibitor-1 (PAI-1), a 45 kDa glycoprotein belonging to the serine protease inhibitor (SERPIN) superfamily . PAI-1 regulates fibrinolysis by inhibiting tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators, thereby stabilizing blood clots . Beyond hemostasis, PAI-1 influences cell migration, tissue remodeling, and immune responses .
Research Applications
SERPINE1 antibodies are utilized across diverse experimental techniques:
Cancer Biology
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Gastric Cancer: SERPINE1 overexpression correlates with advanced tumor grade, metastasis, and poor prognosis. Knockdown reduces proliferation, migration, and invasion via VEGF and IL-6 pathways .
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Triple-Negative Breast Cancer (TNBC): SERPINE1 promotes radioresistance by enhancing DNA double-strand break repair. Inhibitors like tiplaxtinin restore radiation sensitivity in obese mouse models .
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Biomarker Potential: High SERPINE1 expression predicts worse survival in breast, ovarian, and bladder cancers .
Cardiovascular and Metabolic Disorders
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Thrombophilia: Complete PAI-1 deficiency due to SERPINE1 mutations causes abnormal bleeding .
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Ischemic Stroke: Elevated serum anti-SERPINE1 antibodies are linked to hypertension, diabetes, and cardiovascular disease .
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Obesity: Adipocyte-derived SERPINE1 contributes to metabolic syndrome and tumor aggressiveness .
Technical Considerations
Clinical and Therapeutic Implications
Product Specs
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Serpine1 Antibody: A serine protease inhibitor with significant roles in various physiological processes. It primarily inhibits tissue-type plasminogen activator (tPA, PLAT) and urokinase-type plasminogen activator (uPA, PLAU). Its inhibition of tPA is crucial for downregulating fibrinolysis and controlling blood clot degradation. Inhibition of uPA regulates cell adhesion and spreading. Beyond its protease inhibitory function, Serpine1 also modulates cell migration, demonstrably promoting keratinocyte migration during cutaneous wound repair. Further roles include involvement in cellular and replicative senescence, notably impacting alveolar type 2 cell senescence in the lungs. In the context of odontogenesis, Serpine1 regulates cementogenic differentiation of periodontal ligament stem cells and influences odontoblast differentiation and dentin formation.
STRING: 10090.ENSMUSP00000039586
UniGene: Mm.250422
Customer Reviews
Applications : WB
Sample type: Rat visceral adipose tissue
Review: Isolated adipocytes were cultured with or without alamandine for 24 h prior to measuring PAI-1 protein expression.
