SFTPB Antibody
Description
Overview of SFTPB Antibody
The SFTPB antibody is an immunological tool designed to detect surfactant protein B (SP-B), a critical component of pulmonary surfactant encoded by the SFTPB gene. SP-B facilitates surfactant function by reducing alveolar surface tension, preventing lung collapse, and ensuring efficient gas exchange . Deficiencies or mutations in SP-B are linked to severe respiratory conditions, including neonatal respiratory distress and interstitial lung diseases . SFTPB antibodies are used in research and diagnostics to study SP-B expression, localization, and dysfunction in lung pathologies.
Applications of SFTPB Antibody
SFTPB antibodies are utilized across multiple laboratory techniques to investigate SP-B’s role in lung biology and disease. Key applications include:
SP-B in Genetic Lung Disorders
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Surfactant Dysfunction: Over 30 SFTPB mutations cause neonatal respiratory failure due to impaired surfactant processing .
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Hypomorphic Variants: A synonymous SFTPB variant (c.582G>A) causes adult-onset pulmonary fibrosis by reducing mature SP-B expression .
SP-B as a Biomarker in Lung Cancer
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Pro-SFTPB and Risk: Non-detectable or elevated plasma pro-SFTPB levels correlate with increased lung cancer risk (OR = 5.88 and 1.41 per log-unit increase, respectively) .
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Prognostic Utility: Low tumor pro-SFTPB expression predicts early-stage lung adenocarcinoma recurrence (median recurrence time: 49 vs. 77 months) .
SP-B in Acute Lung Injury (ALI)
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Regulation by NAMPT: Knockdown of NAMPT increases SP-B expression, attenuating LPS-induced ALI in mice .
Clinical and Diagnostic Significance
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Genetic Testing: SFTPB antibodies aid in diagnosing hereditary SP-B deficiency via lung biopsy immunostaining .
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Cancer Biomarker: Pro-SFTPB ELISA kits stratify lung cancer risk and monitor therapy response .
Technical Considerations
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Studies have revealed that SP-B(CTERM) positions itself approximately 8 angstroms deeper than phosphate headgroups. This unique topology enables the peptide to facilitate functional lipid structures without inducing micellization under compression. PMID: 22252270
- Research findings suggest that the hydrophobic surfactant protein, SP-B, effectively safeguards alveolar surfactant phospholipids from hydrolysis mediated by various sPLA(2) enzymes within both vesicles (alveolar subphase) and monomolecular films (air-liquid interface). PMID: 22037357
- The hydrophobic surfactant proteins SP-B and SP-C have been shown to promote the formation of bicontinuous inverse cubic phases. PMID: 20409474
- Inactivation of lipid extract surfactant B (SP-B) biophysical activity due to reactive oxygen species appears to involve modifications of a maximum of three amino acids: Trp9, Met29, and Met65. PMID: 17425286
