SGO2 Antibody

Introduction to SGO2 Antibodies

SGO2 antibodies target the shugoshin-like 2 protein (UniProt: Q562F6), a 144.7 kDa nuclear protein encoded by the SGOL2 gene. SGO2 plays essential roles in:

• Protecting centromeric cohesion during mitosis and meiosis

• Facilitating chromosomal passenger complex (CPC) localization

• Regulating heat shock response pathways for cell survival

These antibodies are widely used in techniques such as immunohistochemistry (IHC), Western blotting, and immunofluorescence to study SGO2's expression patterns and functional roles .

Oncogenic Roles in Lung Adenocarcinoma (LUAD)

Data from siRNA experiments validated via Western blot and Transwell assays

Therapeutic Implications

• High SGO2 expression correlates with:

  • Reduced CD4+ T-cell infiltration (p = 0.0032)

  • Increased tumor mutational burden (TMB) (p = 0.017)

  • Enhanced sensitivity to PARP inhibitors (ORR: 42% vs. 18% in low-SGO2 groups)

Clinical Validation Data

• IHC scoring system:

  • Intensity (0–3) × Quantity (0–3) = Composite score

  • Scores >4 predict 3.1× higher mortality risk in glioma patients

• Multicohort validation:

  • TCGA-LUAD: AUC = 0.81 for metastasis prediction

  • GEO-GSE31210: Concordance index = 0.76

Technical Considerations for Use

1. Antigen retrieval: Citrate buffer (pH 6.0) recommended for formalin-fixed tissues

2. Cross-reactivity: Confirm species specificity – murine isoforms share 89% homology

3. Controls: Use siRNA-treated cells or peptide-blocked samples to confirm specificity