SLC45A3 Antibody, Biotin conjugated
Description
Introduction to SLC45A3 Antibody, Biotin Conjugated
The SLC45A3 Antibody, Biotin conjugated is a specialized immunoassay reagent targeting the solute carrier family 45 member 3 (SLC45A3), a transmembrane protein also known as Prostein. This antibody is chemically linked to biotin, enabling high-sensitivity detection in techniques such as immunohistochemistry (IHC), Western blot (WB), and enzyme-linked immunosorbent assay (ELISA) through streptavidin-biotin binding systems. SLC45A3 is associated with prostate cancer and lipid metabolism, making this antibody valuable for oncology and metabolic research .
Immunogen and Specificity
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Immunogen: Synthetic peptides derived from human SLC45A3 (e.g., residues 290–339 or 408–487) .
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Cross-Reactivity: Human, mouse, rat, dog, and guinea pig (varies by product) .
Primary Uses
Research Findings
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Lipid Metabolism: SLC45A3 regulates intracellular glucose and palmitic acid levels in oligodendrocytes, influencing myelin maintenance .
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Prostate Cancer: Overexpression of SLC45A3 correlates with prostate cancer progression, making this antibody critical for biomarker studies .
Product Comparison Across Suppliers
| Supplier | Catalog # | Host | Clonality | Applications | Price (USD) |
|---|---|---|---|---|---|
| Novus Biologicals | NBP3-20790B | Mouse | Monoclonal | IHC-P, WB | $362 |
| Bioss | bs-6568R-Biotin | Rabbit | Polyclonal | WB, IHC | $351 |
| Thomas Scientific | Custom | Rabbit | Polyclonal | ELISA | $166 |
| antibodies-online | ABIN6738106 | Rabbit | Polyclonal | WB | $454.67 |
Validation and Quality Control
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Specificity: Validated via siRNA knockdown in oligodendrocytes and 3T3-L1 adipocytes .
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Performance: Consistent detection in human prostate tissue (IHC) and HepG2 lysates (WB) .
Limitations and Considerations
Product Specs
Target Background
- A novel autoimmune peptide epitope of prostein in prostate cancer has been identified (PMID: 27700103). PMID: 27700103
- CTCF plays a regulatory role in SLC45A3-ELK4 chimeric RNA expression (PMID: 26938874). PMID: 26938874
- Genome-wide association studies have identified rs2153904 in SLC45A3 as the top single nucleotide polymorphism (SNP) associated with log PSA (prostate-specific antigen) levels (PMID: 25434496). PMID: 25434496
- A genome-wide association study identified a novel genetic variant in the SLC45A3 gene associated with serum prostate-specific antigen (PSA) levels in a Chinese population (PMID: 23269536). PMID: 23269536
- SLC45A3 protein expression is downregulated via SLC45A3-ERG fusion in prostate cancer (PMID: 22821757). PMID: 22821757
- The chimeric SLC45A3-ELK4 transcript regulates prostate cancer cell proliferation, and its expression correlates with disease progression (PMID: 22719019). PMID: 22719019
- Sequential immunohistochemical staining with p63 and P501S effectively distinguishes urothelial carcinoma from prostate carcinoma (PMID: 21777423). PMID: 21777423
- Concurrent TMPRSS2 and SLC45A3 rearrangements have been reported in the same prostate cancer tumor focus (PMID: 20118910). PMID: 20118910
- T cells recognizing naturally processed prostein epitopes have been expanded and characterized from healthy donors, revealing differences in avidity between male and female-derived T cells (PMID: 15048720). PMID: 15048720
- Prostein is widely expressed in prostatic tissues, regardless of grade or metastatic status, but not in a large panel of other normal and malignant tissues (PMID: 15176054). PMID: 15176054
- P501S is a sensitive and specific marker for prostate tissue, useful in combination with prostein/prostate-specific antigen for detecting prostatic origin of metastatic carcinomas (PMID: 17721190). PMID: 17721190
- An SLC45A3:ETV5 gene fusion has been identified in prostate cancer (PMID: 18172298). PMID: 18172298
- Intronic miRNAs from tissue-specific transcripts significantly contribute to cellular gene expression and phenotype (PMID: 18193184). PMID: 18193184
- Most ERG-overexpressing prostate cancers contain hormonally regulated TMPRSS2-ERG, SLC45A3-ERG, or NDRG1-ERG fusions (PMID: 19649210). PMID: 19649210
- SLC45A3-ELK4 is a recurrent prostate cancer-specific RNA chimeric transcript without a detectable DNA aberration (PMID: 19136943). PMID: 19136943
Q&A
Basic Research Questions
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What is SLC45A3 and what is its biological function?
SLC45A3 (Solute Carrier Family 45 Member 3) is a transmembrane protein that functions as a proton-associated sugar transporter. It primarily facilitates sucrose transport but may also transport glucose and fructose . SLC45A3 plays several important biological roles:
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Regulates intracellular glucose concentrations in oligodendrocytes
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Contributes to neutral lipid accumulation when overexpressed
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Involved in myelin protein maintenance and oligodendrocyte morphology
The protein is also known by several other names including Prostein and Prostate cancer-associated protein 6 (PCANAP6) , reflecting its enriched expression in prostate tissue.
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What are the common applications for SLC45A3 Antibody, Biotin conjugated?
Based on manufacturer specifications, SLC45A3 Antibody, Biotin conjugated is primarily validated for:
Application Validated Notes ELISA Yes Primary application IHC-P Potential Requires optimization Western Blot Variable Depends on specific antibody clone The biotin conjugation allows for enhanced detection sensitivity in immunoassays through the strong biotin-streptavidin interaction, making it particularly useful for signal amplification in samples with low target expression .
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What are the recommended storage and handling conditions for SLC45A3 antibodies?
For optimal stability and performance of SLC45A3 antibodies including biotin-conjugated formats:
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Typical buffer composition: 50% Glycerol, 0.01M PBS, pH 7.4 with preservatives such as 0.03% Proclin 300
When handling the antibody for experiments, maintain cold chain conditions and follow manufacturer's dilution recommendations for specific applications to ensure optimal performance .
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What tissues and species show reactivity with SLC45A3 antibodies?
SLC45A3 antibodies show reactivity primarily with:
Species Reactivity Tissue Expression Human High Prostate (high), Oligodendrocytes, Myelin Mouse Variable Brain, Oligodendrocytes, Liver Rat Limited data Nervous system tissues SLC45A3 shows particularly strong expression in prostate tissues, making it a valuable marker in prostate cancer research. It's also enriched in oligodendrocytes and myelin fractions of the brain, supporting its role in myelin maintenance .
