SLIT3 Antibody, FITC conjugated
Description
Introduction to SLIT3 Antibody, FITC Conjugated
The SLIT3 Antibody, FITC conjugated is a fluorescently labeled rabbit polyclonal antibody designed to detect the Slit Homolog 3 (SLIT3) protein, a glycoprotein involved in axon guidance, angiogenesis, and tissue remodeling. FITC (fluorescein isothiocyanate) conjugation enables visualization under fluorescence microscopy, making it ideal for immunofluorescence studies.
Mechanism of Action
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Fluorescence Detection: FITC emission at 515 nm allows precise localization of SLIT3 in cellular or tissue sections.
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Cross-Reactivity: Limited to human SLIT3, though homology exists with other species (e.g., mouse, rat) .
Angiogenesis and Vascular Studies
SLIT3 is a potent angiogenic factor that promotes endothelial cell proliferation and migration. The FITC-conjugated antibody has been used to:
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Visualize SLIT3 expression in endothelial cells and vascular smooth muscle cells (VSMCs) .
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Track SLIT3 secretion in co-culture systems (e.g., endothelial cells and fibroblasts) .
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Monitor SLIT3-mediated angiogenesis in models of tissue repair or cancer .
Fibrosis and Tissue Remodeling
SLIT3 regulates fibrillar collagen synthesis in fibroblasts. The antibody aids in:
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Studying SLIT3’s role in myocardial fibrosis and ventricular remodeling .
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Assessing therapeutic potential of targeting SLIT3 in fibrotic diseases (e.g., pulmonary fibrosis, liver cirrhosis) .
Neurovascular Crosstalk
In brown adipose tissue (BAT), SLIT3 fragments coordinate angiogenesis and sympathetic innervation. The FITC-conjugated antibody enables:
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Mapping SLIT3 expression in adipocyte progenitors and endothelial cells during cold-induced thermogenesis .
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Analyzing proteolytic processing of SLIT3 into functional fragments .
Comparative Analysis of SLIT3 Antibodies
The table below compares the FITC-conjugated antibody with other SLIT3 antibodies from diverse sources:
Angiogenic Signaling
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EC Proliferation: SLIT3 stimulates endothelial cell mitosis at concentrations comparable to VEGF .
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Vascular Expansion: SLIT3-deficient mice exhibit reduced CD31hiEMCNhi endothelium, critical for osteogenesis and tissue repair .
Fibrotic Pathways
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Collagen Synthesis: Global SLIT3 deficiency reduces type I/III collagen expression and alleviates pressure overload-induced cardiac fibrosis .
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Therapeutic Target: SLIT3 inhibition may mitigate adverse ventricular remodeling in heart failure .
Neurovascular Coordination
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Overexpression of Slit3 exhibits tumor suppressive effects in breast cancer. PMID: 26542734
- Research indicates that silencing Slit3 promotes proliferation, migration, and invasion of A549 cells and induces epithelial-mesenchymal transition, suggesting that Slit3 functions as a tumor suppressor in lung carcinoma. PMID: 26045181
- Estrogen-dependent expression of SLIT3 may play a crucial role in regulating nerve-vessel interactions within the complex microenvironment of endometriosis lesions. PMID: 25051436
- SLIT3 expression increases with labor, and studies on both amnion and myometrium describe a pro-inflammatory effect of SLIT3 in these tissues. PMID: 24286238
- High SLIT3 expression is associated with high-grade gliomas. PMID: 24158112
- SLIT3-ROBO4 activation promotes vascular network formation in human engineered tissue and angiogenesis in vivo. PMID: 24090675
- Findings implicate the involvement of miR-218-2 and its host gene SLIT3 in thyroid cancer cell invasion, migration, and proliferation. PMID: 23720784
- Slit3 treatment enhanced the in vivo homing efficiency of CD34 HSPCs to the BM in NOD/SCID mice, while Slit3-exposed HSPC migration in vitro was inhibited. These results support a role for Slit3 in human HSPC migration in vitro and homing in vivo. PMID: 22614124
- SLIT3 duplication on 5q35.1 predisposes to major depressive disorder. PMID: 21152026
- The chemorepellent Slit3 promotes monocyte migration. PMID: 21078908
- Slit3 inhibits Robo3-induced invasion of synovial fibroblasts in rheumatoid arthritis. PMID: 20298552
- Multiple variants in SLIT3 tend to associate with schizophrenia susceptibility in the Chinese Han population. PMID: 15231749
- Data suggest that Slit proteins play a crucial role in guiding dorsally projecting cranial motoneurons and facilitating their neural tube exit. PMID: 16162649
- In fetal and embryonic stem cell cultures, Slit-3 inhibited neurite outgrowth. PMID: 16840550
- SLIT3 functions to promote angiogenesis in coordinating organogenesis during embryonic development. PMID: 19741192
