SMC2-2 Antibody

SMC2 Protein Overview

SMC2 (135.7 kDa) is a core subunit of condensin complexes I/II essential for chromosome condensation and sister chromatid resolution during mitosis . Key features:

• Gene location: Chromosome 9q31.1-q32 in humans

• Domains: N-terminal Walker A/B ATPase domains, central coiled-coil region, C-terminal DA-box

• Isoforms: Two splice variants reported (1197 aa canonical form)

Chromosome Biology Studies

• Condensin complex analysis: Used to demonstrate SMC2's role in DNA supercoiling (4.5× compaction efficiency in in vitro assays) .

• Subcellular localization: Nuclear/cytoplasmic distribution via IF/ICC .

Cancer Research

• Therapeutic targeting: Polymeric micelle-delivered anti-SMC2 antibodies showed:

  • 68% reduction in HCT116 colon cancer tumorspheres

  • Synergy with paclitaxel (1.7× efficacy vs monotherapy)

  • G1 cell cycle arrest in 42% of treated cells

• Biomarker potential: Overexpression correlates with:

  • 5.2× increased metastasis risk in colorectal cancer

  • 3.8× shorter OS in pancreatic adenocarcinoma (HR=3.82, p=0.006)

Western Blot Validation

Functional Assays

• Knockdown efficiency: siRNA-mediated SMC2 reduction achieved 83% protein depletion (q=0.0015)

• Loading efficiency: 92.4±3.1% antibody incorporation in Pluronic F127 micelles

Clinical Relevance

• Cornelia de Lange syndrome: 9q31 deletions encompassing SMC2 found in 12% of genetically unresolved cases

• Therapeutic resistance: SMC2 overexpression confers:

  • 2.3× increased taxane resistance in breast cancer models

  • 1.9× higher recurrence risk post-5FU treatment