SMIM14 Antibody

Applications in Research

SMIM14 Antibodies are employed in multiple techniques to analyze protein expression, localization, and function:

Western Blotting (WB)

• Purpose: Detect SMIM14 in lysates or membrane fractions.

• Example: Novus Biologicals’ NBP2-83564 antibody detects SMIM14 in COLO205 whole-cell lysates at 1.0 µg/ml .

• Optimal Dilution: 1:1000–1:2000 (WB) .

Flow Cytometry (FACS)

• Purpose: Analyze SMIM14 expression in hematopoietic cells or erythroid progenitors.

• Example: ABIN2215935 (unconjugated) and ABIN1936663 (FITC-conjugated) are validated for FACS .

Immunohistochemistry (IHC) and Immunofluorescence (IF)

• Purpose: Localize SMIM14 in tissue sections or cultured cells.

• Example: PACO40198 detects SMIM14 in human placenta (IHC) and HeLa cells (IF) .

ELISA

• Purpose: Quantify SMIM14 levels in bodily fluids or cell supernatants.

• Example: ABIN2215935 and PACO40198 are validated for ELISA at 1:2000–1:10,000 dilution .

Role in Erythropoiesis and Hematological Disorders

SMIM14 is implicated in red blood cell maturation. Dysregulation may contribute to anemia or erythroid-related diseases . Antibodies like PACO40198 enable studies linking SMIM14 expression to hematopoietic stem cell differentiation .

ER Retention and Transmembrane Domain

SMIM14 localizes to the ER membrane, where its transmembrane domain (AA 51–70) may mediate ER retention . Antibodies targeting the C-terminal region (e.g., ABIN2215935) are ideal for studying ER-associated SMIM14 .

Gene Structure and Expression

The SMIM14 gene spans 92,567 base pairs on chromosome 4p14, with five exons and three transcript variants . Key features include:

Table 1: Promoter Regions of SMIM14

High Expression Tissues: Liver, adrenal gland, colon, and prostate .

Table 2: SMIM14 Transcript Variants

Critical Considerations for Antibody Selection

• Epitope Specificity: C-terminal antibodies (e.g., ABIN2215935) may better detect ER-associated SMIM14, while N-terminal variants (e.g., PACO40198) are suited for cytoplasmic or membrane-bound forms .

• Cross-Validation: Use multiple antibodies (e.g., WB and IHC) to confirm results due to potential epitope masking .

References

1. Antibodies-online. SMIM14 Antibody (C-Term). ABIN2215935.

2. Wikipedia. Small integral membrane protein 14. SMIM14.

3. Assay Genie. SMIM14 Antibody (PACO40198). PACO40198.

4. Abcepta. SMIM14 Antibody (C-term). AP5437b.

5. Bio-Techne. SMIM14 Antibody (NBP2-83564). NBP2-83564.

6. Antibodies-online. SMIM14 Antibody FITC (ABIN1936663). ABIN1936663.