sumo3l Antibody

Definition and Biological Role of SUMO3

SUMO3 (Small Ubiquitin-like Modifier 3) is a post-translational modifier protein involved in sumoylation, regulating processes like nuclear transport, DNA repair, and transcriptional regulation . Unlike ubiquitination, sumoylation does not degrade proteins but modulates their activity or localization .

Key Features of SUMO3:

SUMO3 Antibody Applications and Validation

SUMO3 antibodies are critical for detecting SUMO3 conjugates in research. Below is a comparative analysis of commercially available antibodies:

Table 1: Key SUMO3 Antibodies and Their Properties

Notes:

• ab203570 demonstrates nuclear localization in MCF7 cells .

• AF2959 detects SUMO3 at ~11 kDa in A549 and DU145 cell lines .

• Cross-reactivity with SUMO2/4 is common due to high sequence homology .

Research Findings on Antibody Specificity

A 2022 study evaluated 24 anti-SUMO monoclonal antibodies (MAbs), revealing critical insights :

Table 2: Antibody Performance in Key Assays

Critical Observations:

• 52% of SUMO2/3 MAbs exhibited cross-reactivity with SUMO4 .

• Only 33% of antibodies reliably detected SUMO3 in stress-induced sumoylation assays .

Technical Considerations for SUMO3 Detection

• Sample Preparation: Use reducing conditions (e.g., β-mercaptoethanol) to maintain SUMO3 integrity .

• Controls: Include peptide competition assays to validate specificity .

• Storage: Antibodies require -70°C storage and avoidance of freeze-thaw cycles .

Challenges and Limitations

• Cross-Reactivity: High homology among SUMO2/3/4 complicates isoform-specific detection .

• Polymer Detection: Many MAbs fail to recognize SUMO2/3 polymeric chains .