SMR7 Antibody
Description
Functional Role of SMR7
SMR7 belongs to the SIAMESE-RELATED family of CKIs, which regulate cell cycle progression and DNA damage checkpoints in plants . Key findings include:
-
Checkpoint Regulation: SMR7 is transcriptionally activated by DNA damage agents like hydroxyurea (HU) and bleomycin. Knockout SMR7 plants exhibit impaired cell cycle arrest under replication stress, indicating its role in maintaining genomic stability .
-
ROS-Dependent Activation: Unlike typical replication-stress responses mediated by ATR kinase, SMR7 induction primarily depends on ATM kinase and the transcription factor SOG1, linking it to oxidative stress pathways .
Antibody Applications and Validation
While direct data on SMR7 antibody validation is limited in the provided sources, insights can be extrapolated from related plant studies:
Table 1: Hypothetical Applications of SMR7 Antibody
| Application | Purpose | Example Technique |
|---|---|---|
| Protein Localization | Detect SMR7 expression in plant tissues under DNA damage | Immunohistochemistry |
| Western Blot | Quantify SMR7 levels in Arabidopsis mutants or stress-treated plants | SDS-PAGE/Immunoblotting |
| Functional Studies | Assess SMR7 interaction with cell cycle regulators (e.g., CDKs) | Co-Immunoprecipitation |
Research Context and Gaps
-
Transcriptional Induction: SMR7 mRNA levels increase 5.68-fold in Arabidopsis exposed to bleomycin, with similar induction under γ-irradiation .
-
Genetic Interactions: SMR7 works redundantly with SMR4 and SMR5 to enforce cell cycle arrest, as single knockouts show partial checkpoint defects .
Comparative Analysis with Mammalian Systems
Though unrelated mechanistically, Smad7 (a TGF-β signaling inhibitor in humans) shares nomenclature similarities. For clarity:
Table 2: SMR7 vs. Smad7
Future Directions
-
Antibody Development: Specific monoclonal antibodies against SMR7 could enable precise localization and interaction studies.
-
Translational Studies: Explore SMR7 homologs in crops to engineer stress-resistant varieties.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- SMR5 and SMR7 are components of a signaling cascade that triggers a cell cycle checkpoint in response to reactive oxygen species (ROS)-induced DNA damage. PMID: 24399300
