smarcad1a Antibody

Antody Overview

SMARCAD1a Antibody specifically targets the SMARCAD1 protein, an ATP-dependent DNA helicase involved in resolving R-loops at replication forks , maintaining heterochromatin integrity , and suppressing tumorigenesis . In zebrafish, smarcad1a and smarcad1b are homologs of human SMARCAD1, with smarcad1a showing tumor suppressor activity in malignant peripheral nerve sheath tumors (MPNSTs) .

3.1. DNA Replication and Genome Stability

• SMARCAD1 resolves R-loops at active replication forks to prevent DNA damage . Antibodies like #12458 enable detection of SMARCAD1 localization at replication sites .

• Mutant SMARCAD1 cells accumulate R-loops and exhibit replication-associated mutagenesis, detectable via Western blotting and immunofluorescence .

3.2. Tumor Suppression

• In zebrafish, smarcad1a loss accelerates MPNST tumorigenesis in tp53-mutant backgrounds . Antibodies validate SMARCAD1 depletion in tumor models (e.g., reduced protein levels in MPNST cell lines) .

• SMARCAD1 deficiency correlates with impaired DNA double-strand break repair, measurable via pH2AX staining .

3.3. Immune Evasion Mechanisms

• SMARCAL1 (a related helicase) regulates PD-L1 expression and innate immune evasion . While distinct from SMARCAD1, shared methodologies (e.g., ChIP, WB) apply to both targets .

Critical Research Findings

• Tumorigenesis: smarcad1a mutants (sa1299, p403) in zebrafish show 2–3× faster MPNST growth than controls .

• DNA Repair: SMARCAD1 knockdown in human MPNST cells delays pH2AX resolution post-irradiation, confirming its role in DNA repair .

• Therapeutic Potential: SMARCAD1-mutant cells exhibit sensitivity to PARP inhibitors, suggesting clinical relevance .

Methodological Considerations

• Western Blotting: Use 4–20% gradient gels for optimal separation of the 150 kDa SMARCAD1 protein .

• Immunoprecipitation: Combine with anti-biotin HRP (#7075) to detect protein markers during IP .

• Controls: Include isotype-matched controls (e.g., Rabbit IgG #2729) to validate specificity .