SMT2 Antibody

Target Protein: SMTNL2 (Smoothelin-Like 2)

SMTNL2 is a 50 kDa protein encoded by the SMTNL2 gene (NCBI Gene ID: 342527). It is expressed in smooth muscle tissues and plays roles in:

• Modulating myosin phosphatase activity .

• Regulating calcium sensitivity in smooth muscle contraction .

• Involvement in metabolic pathways, including glucose uptake and insulin signaling .

3.1. Tissue and Cell Line Reactivity

The antibody detects SMTNL2 in:

• Mouse small intestine tissue .

• Human cell lines: MCF-7 (breast cancer), COLO 320 (colorectal cancer), and SGC-7901 (gastric cancer) .

3.2. Key Findings in Research

• Smooth Muscle Function: SMTNL2 modulates myosin light-chain phosphatase, influencing smooth muscle tone .

• Cancer Relevance: Elevated SMTNL2 expression correlates with tumor progression in colorectal and gastric cancers .

• Subcellular Localization: Predominantly cytoplasmic, with dynamic redistribution during cellular stress .

4.1. Western Blot

• Detects a ~68–70 kDa band in human, mouse, and rat lysates (Fig. 1A) .

• Positive controls: COLO 320 (colorectal cancer) and MCF-7 cells .

4.2. Immunofluorescence

• Localizes SMTNL2 to the cytoplasm in MCF-7 cells (Fig. 1B) .

4.3. Immunoprecipitation

• Efficiently pulls down SMTNL2 from COLO 320 cell lysates .

Comparative Analysis of Antibody Performance

Limitations and Considerations

• Post-Translational Modifications: Observed molecular weight discrepancies (68–70 kDa vs. predicted 50 kDa) require careful interpretation .

• Cross-Reactivity: Untested in non-mammalian systems .