SNF8 Antibody, FITC conjugated
Description
FITC Conjugation Process
The conjugation of FITC to antibodies involves covalent binding to lysine residues, typically achieving 3–6 FITC molecules per antibody to balance brightness and solubility . Key considerations:
-
Optimization: Titration experiments are critical to avoid over-conjugation, which can reduce binding affinity .
-
Stability: FITC-conjugated antibodies are light-sensitive; prolonged exposure leads to fluorescence loss .
Western Blotting
-
Dilution: 1:500–1:3000 (optimized for signal-to-noise ratio) .
-
Example: Detection of SNF8 in HeLa cell lysates (30 μg/lane) at 1:1000 dilution .
Immunohistochemistry (IHC)
-
Dilution: 1:50–1:500; antigen retrieval with TE buffer (pH 9.0) or citrate buffer (pH 6.0) .
-
Example: Staining human colon carcinoma tissue (1:250 dilution) .
Immunofluorescence (IF)
-
Dilution: 1:50–1:500 for IF/ICC; used in whole-mount zebrafish embryo studies (1:200) .
-
Note: FITC excitation at 488 nm (argon laser) enables compatibility with fluorescence microscopy .
Research Findings
Critical Considerations
-
Cross-reactivity: FITC-conjugated antibodies may exhibit species-specific binding (e.g., C3c complement in multiple mammals) .
-
Storage: Sodium azide preservative must be removed before conjugation to prevent reaction interference .
-
Quality Control: FITC-conjugated antibodies require rigorous testing for specificity and fluorescence stability .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research indicates an unexpected role for EAP30 in the nuclear IRF3-dependent innate antiviral response. PMID: 29084253
- Collectively, these findings suggest an active role for ESCRT-II and CHMP6 in ESCRT-mediated abscission. PMID: 25232011
- Modulating SNF8 expression levels alters the TRPC6 channel current and can modulate the activation of NFAT-mediated transcription downstream of gain-of-function mutant TRPC6. PMID: 23171048
- Mammalian ESCRTII is composed of hVps25p, hVps22p, and hVps36p and may be redundant, cargo-specific, or not required for protein sorting at the multivesicular body. PMID: 16371348
- Evidence suggests that multiple pathways exist for protein sorting and multivesicular body vesicle formation in human cells and that human immunodeficiency virus type 1 does not utilize an ESCRT-II-dependent pathway to exit the cell. PMID: 16973552
- Vps22 plays a significant role in ligand-induced EGFR and CXCR4 turnover; termination of EGF signaling occurs before ESCRT-II engagement. PMID: 17714434
