SNX12 Antibody, Biotin conjugated

Production and Characterization

SNX12 antibodies are typically generated using recombinant proteins or synthetic peptides. Biotin-conjugated variants undergo additional chemical modification to attach biotin molecules, enabling downstream detection with streptavidin-linked enzymes (e.g., HRP) or fluorophores.

Key Features:

For example, Novus Biologicals’ NBP2-74263B (mouse monoclonal) and LSBio’s LS-C470311 (rabbit polyclonal) are validated for specificity and cross-reactivity in human, mouse, and rat samples .

Applications

Biotin-conjugated SNX12 antibodies are employed in diverse assays, with optimized dilutions for sensitivity and specificity.

Recommended Dilutions by Application:

Role of SNX12 in Endosomal Trafficking

SNX12 is homologous to SNX3 and interacts with 3-phosphoinositides (PtdIns3P) to localize to early endosomes. Overexpression disrupts multivesicular body (MVB) maturation, impairing lysosomal degradation . Key insights include:

• Membrane Binding: Dependent on PX domain interaction with PtdIns3P; mutation of Arg71 abolishes binding .

• Retromer Interaction: Co-localizes with retromer components (Vps35, Vps26), suggesting a role in retrograde transport .

Biotin Interference in Assays

High biotin levels in samples (e.g., egg yolk) can cross-react with streptavidin, causing false positives. This is mitigated by optimizing blocking buffers or using non-biotin-based detection systems .

Troubleshooting and Best Practices

1. Avoid Freeze-Thaw Cycles: Repeated freezing degrades antibody activity .

2. Optimize Blocking: Use 10% normal goat serum to reduce non-specific binding .

3. Biotin Interference: Confirm sample biotin levels before ELISA/WB to prevent false signals .