SNX5 Antibody
Description
SNX5 Antibody Overview
SNX5 antibody (e.g., Proteintech 17918-1-AP) is a polyclonal rabbit IgG antibody targeting the SNX5 protein, which plays roles in endosomal sorting, actin remodeling, and antigen presentation. Key properties include:
| Property | Detail |
|---|---|
| Target | SNX5 (sorting nexin 5) |
| Host Species | Rabbit |
| Reactivity | Human, mouse, rat |
| Molecular Weight | ~47 kDa (observed) |
| Applications | WB, IP, IHC, ELISA |
| Immunogen | SNX5 fusion protein (Ag12330) |
| Storage | -20°C in PBS with 0.02% sodium azide and 50% glycerol |
| UniProt ID | Q9Y5X3 |
This antibody has been validated in diverse samples, including HEK-293T cells, kidney tissues, and immune cells like Jurkat and K-562 .
2.1. Antigen Presentation in Immune Cells
SNX5 regulates actin dynamics and lysosomal trafficking in B cells, critical for immune synapse formation and antigen extraction :
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Membrane remodeling: SNX5 localizes to plasma membrane ruffles in resting B cells, facilitating antigen screening. Upon activation with immobilized antigens, SNX5 accumulates at the immune synapse to promote actin-dependent spreading .
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Lysosomal integrity: SNX5-silenced B cells exhibit enlarged lysosomes, impairing antigen extraction and presentation .
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Retromer complex: SNX5 interacts with retromer components to retrieve cargo from endosomes to the Golgi, affecting B-cell activation and antibody production .
2.2. Oncogenic Role in Cancer
SNX5 promotes tumor growth and therapy resistance in head and neck squamous cell carcinoma (HNSCC):
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Tumor progression: SNX5 knockdown reduces colony formation in soft agar assays and decreases tumor volume in xenograft models .
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NOTCH1 signaling: SNX5 stabilizes cleaved NOTCH1 and modulates Mcl-1 expression, enhancing cell survival .
2.3. Macropinocytosis in Macrophages
SNX5 is essential for macropinosome biogenesis in primary macrophages, enabling efficient uptake and processing of soluble antigens (e.g., ovalbumin) .
Protocol Recommendations
4.1. Immune System Studies
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SNX5 depletion disrupts lysosomal recruitment to synaptic membranes, reducing antigen extraction by 60% in B cells .
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In macrophages, SNX5 loss decreases macropinosome formation by 70%, impairing soluble antigen processing .
4.2. Cancer Studies
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High SNX5 expression correlates with poor prognosis in HNSCC .
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SNX5 knockdown reduces cleaved NOTCH1 levels by 40%, sensitizing cells to apoptosis .
Validation and Citations
This antibody has been cited in 13+ publications, including studies on:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Elevated SNX5 expression is associated with alphavirus infections. PMID: 29743363
- Sorting nexin 5 (SNX5), a component of the retromer and part of the retrograde transport pathway, interacts with UL35 proteins. Binding of UL35 proteins to SNX5 is essential for efficient viral replication and transport of the most abundant cytomegalovirus glycoprotein B to the cytoplasmic viral assembly center. PMID: 29444945
- The Chlamydia trachomatis IncE binding site is unique to human SNX5 and related family members SNX6 and SNX32. PMID: 28226239
- Sorting nexin 5 and dopamine d1 receptor regulate the expression of the insulin receptor in human renal proximal tubule cells. PMID: 25825816
- Internalization of agonist-activated D1R is regulated by both SNX5 and GRK4, and SNX5 is crucial for the recycling of the receptor to the plasma membrane. PMID: 23195037
- Data suggest that caspase-2 is induced by sorting nexin 5 (SNX5) in papillary thyroid carcinoma. PMID: 22486813
- SNX5 is localized to a subdomain of the early endosomes and is recruited to the cell membrane following EGF stimulation. PMID: 15561769
- SNX5 and SNX6 may be functional equivalents of Vps17p in mammalian retromer. PMID: 17148574
- DOCK180 regulates CI-MPR trafficking via SNX5, a function independent of its guanine nucleotide exchange factor activity toward Rac1. PMID: 18596235
