SPARCL1 Mouse

SPARC Like 1 Mouse Recombinant
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Cat. No.
BT3085
Source
Sf9, Baculovirus cells.
Synonyms

Sparcl1, Ecm2, hevin, mast9, Sc1, SPARC-like protein 1, Extracellular matrix protein 2, Matrix glycoprotein Sc1.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Tissue Distribution and Expression

SPARCL1 is expressed in multiple tissues but absent in the liver :

TissueExpression LevelKey Isoforms
BrainHighFull-length (130 kDa) and truncated forms
KidneyModerateDominant 55 kDa C-terminal fragment
Heart/LungModerateFull-length and truncated forms
LiverNoneN/A

  • Truncated forms (e.g., 55 kDa in mouse kidney, 25 kDa in human tumors) correlate with tissue-specific functions and disease states .

Anti-Adhesive and Neuronal Roles

  • Inhibits cell adhesion and spreading on collagen-rich substrates, terminating neuronal migration during development .

  • Promotes excitatory synapse formation by increasing AMPA/NMDA receptor ratios independently of neurexins/neuroligins .

Disease Associations

Disease ModelSPARCL1 RoleMechanism
Prostate CancerDownregulated in high-grade tumors and metastases; suppresses RHOC-mediated cell migration Loss enhances epithelial invasion and metastasis
Viral PneumoniaEndothelial overexpression induces pro-inflammatory macrophages, exacerbating lung injury Drives M1-like macrophage polarization
Nonalcoholic Steatohepatitis (NASH)Promotes hepatic inflammation and fibrosis via macrophage accumulation Increases hepatocyte death and collagen deposition

Recombinant SPARCL1 Mouse Protein

  • Production: Expressed in Sf9 Baculovirus cells as a 71.7 kDa His-tagged glycoprotein .

  • Applications: Used in adhesion assays, synaptic activity studies, and collagen-binding experiments .

Antibodies

  • SPARCL1 (F3V2I) Mouse mAb: Detects endogenous SPARCL1 at ~140 kDa via Western blot (1:1000 dilution) and immunofluorescence .

Clinical and Therapeutic Insights

  • Biomarker Potential: Reduced SPARCL1 expression in prostate cancer independently predicts metastatic recurrence .

  • Therapeutic Target: Blocking SPARCL1 in endothelial cells ameliorates viral pneumonia severity , while its inhibition in NASH models reduces liver fibrosis .

Product Specs

Introduction
SPARC-like protein 1, or SPARC1, is an anti-adhesive protein structurally similar to the SPARC protein. It is highly expressed in various tissues, including the brain, lungs, kidneys, and heart, but not in the liver. SPARC1 inhibits the spreading and adhesion of numerous substrates. This protein is believed to trigger anti-adhesive signaling that halts neuronal migration during development and in response to trauma, consistent with its production by glial and neuronal cells.
Description
Recombinant SPARCL1 Mouse, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 642 amino acids (17-650 a.a) with a molecular mass of 71.7kDa. It is fused to an 8 amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
SPARCL1 protein solution (0.25mg/ml) in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 90.0% purity as determined by SDS-PAGE.
Synonyms

Sparcl1, Ecm2, hevin, mast9, Sc1, SPARC-like protein 1, Extracellular matrix protein 2, Matrix glycoprotein Sc1.

Source
Sf9, Baculovirus cells.
Amino Acid Sequence

IPTSTRFLSD HSNPTTATLV TPEDATVPIA GVEATADIEN HPSDKAEKPS ALNSEEETHE
QSTEQDKTYS FEVDLKDEED GDGDLSVDPT EGTLTLDLQE GTSEPQQKSL PENGDFPATV
STSYVDPNQR ANITKGKESQ EQPVSDSHQQ PNESSKQTQD LKAEESQTQD PDIPNEEEEE
EEDEEEEEEE EPEDIGAPSD NQEEGKEPLE EQPTSKWEGN REQSDDTLEE SSQPTQISKT
EKHQSEQGNQ GQESDSEAEG EDKAAGSKEH IPHTEQQDQE GKAGLEAIGN QKDTDEKAVS
TEPTDAAVVP RSHGGAGDNG GGDDSKHGAG DDYFIPSQEF LEAERMHSLS YYLKYGGGEE
TTTGESENRR EAADNQEAKK AESSPNAEPS DEGNSREHSA GSCTNFQCKR GHICKTDPQG
KPHCVCQDPE TCPPAKILDQ ACGTDNQTYA SSCHLFATKC RLEGTKKGHQ LQLDYFGACK
SIPACTDFEV AQFPLRMRDW LKNILMQLYE PNPKHGGYLN EKQRSKVKKI YLDEKRLLAG
HPIELLLRD FKKNYHMYVY PVHWQFNELD QHPADRILTH SELAPLRASL VPMEHCITRF
FEECDPNKDK HITLKEWGHC FGIKEEDIDE NLLFLEHHHH HH

Q&A

Basic Research Questions

  • What experimental models are most appropriate for studying SPARCL1 in murine systems?

    • CRISPR-Cas9 knockout models: Used to generate endothelial cell (EC)-specific Sparcl1-KO mice, enabling tissue-specific functional studies (e.g., viral pneumonia outcomes) .

    • Tamoxifen-inducible Cre systems: Allow temporal control of Sparcl1 deletion/overexpression in adult mice .

    • 3D prostasphere assays: Employed to study SPARCL1's anti-invasive effects in prostate cancer using primary murine/human epithelial cells .

  • How is SPARCL1 expression detected and quantified in mouse tissues?

    • Western blotting: Validates SPARCL1 protein levels in lung homogenates (e.g., EC Sparcl1-OE mice) .

    • RNA sequencing (RNA-seq): Profiles macrophage polarization states in Sparcl1-modulated mice post-influenza infection .

    • Flow cytometry: Quantifies M1/M2-like macrophage subsets (CD206, CD11b, F4/80 markers) in lung/bone marrow-derived macrophages (BMDMs) .

  • What are the baseline physiological roles of SPARCL1 in mice?

    • Anti-adhesive signaling: Disrupts cell-matrix adhesion via collagen binding, critical in prostate development and neuronal migration .

    • Immune modulation: Under homeostasis, SPARCL1 does not alter macrophage polarization but exacerbates inflammatory responses during injury .

Advanced Research Questions

  • How does SPARCL1 differentially regulate disease outcomes in viral pneumonia vs. metabolic disorders?

    ConditionModelSPARCL1 ModulationKey OutcomeSource
    Viral pneumoniaEC Sparcl1-KO miceKnockoutReduced weight loss, improved survival
    NASHHigh-fat diet miceChronic overexpressionIncreased hepatic inflammation, fibrosis

    • Mechanistic insight: In pneumonia, SPARCL1 promotes M1-like macrophage polarization via TLR4/NF-κB signaling. In NASH, it enhances hepatic macrophage infiltration and hepatocyte death .

  • How to reconcile contradictory findings on SPARCL1’s role in cancer vs. inflammatory diseases?

    • Prostate cancer: SPARCL1 loss increases metastasis via RHOC-mediated migration .

    • Viral pneumonia: SPARCL1 overexpression amplifies inflammation, worsening outcomes .

    • Resolution: Tissue-specific context and disease stage dictate SPARCL1’s pro- or anti-pathogenic roles. Use conditional knockout/overexpression models to isolate organ-specific effects.

  • What methodologies resolve SPARCL1’s dual role in macrophage polarization?

    • In vitro BMDM treatment: Recombinant SPARCL1 (5–20 µg/mL) induces NF-κB phosphorylation and TNF-α/IL-6 secretion, mimicking LPS-induced M1 polarization .

    • In vivo cytokine profiling: BALF analysis post-infection reveals elevated TNF-α/IL-1β in EC Sparcl1-OE mice by day 20 .

Technical and Data Analysis Challenges

  • How to address species-specific SPARCL1 expression variability in translational studies?

    • Key finding: Murine SPARCL1 shares 89% amino acid identity with rats but ≤67% with humans, impacting collagen-binding affinity and anti-adhesive functions .

    • Recommendation: Validate findings in humanized mouse models or parallel human cell-line experiments.

  • What controls are critical for interpreting SPARCL1 knockout/overexpression phenotypes?

    • Baseline metrics: Monitor body weight, oxygen saturation, and BALF cell counts in EC Sparcl1-KO/OE mice under homeostasis .

    • Off-target checks: Use RNA-seq to confirm absence of compensatory SPARC family gene upregulation .

Data Contradiction Analysis

  • Why does SPARCL1 enhance synaptogenesis in neurons but inhibit adhesion in epithelial cells?

    • Neuronal context: SPARCL1 boosts excitatory synapse formation via neurexin/neuroligin interactions, independent of adhesion pathways .

    • Epithelial context: Binds collagen to disrupt α2β1-integrin/RHOC signaling, reducing migration .

  • How to validate SPARCL1’s role in macrophage polarization across studies?

    • Consensus markers: Use CD206 (M2) and TNF-α/IL-6 (M1) across models.

    • Discrepancy note: Sparcl1-KO reduces M1 polarization in pneumonia but increases it in NASH , highlighting disease-specific microenvironmental crosstalk.

Product Science Overview

Structure and Characteristics

SPARCL1 is an anti-adhesive protein widely expressed in tissues such as the brain, heart, lung, muscle, and kidney, but notably absent in the liver . The protein contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation . The recombinant mouse SPARCL1 protein is typically produced in cell lines such as NS0 or HEK293 cells and is often tagged with a polyhistidine tag for purification purposes .

Function and Biological Role

SPARCL1 plays a significant role during embryogenesis and tissue remodeling . It is especially prominent in the brain and vasculature. The protein is known for its anti-adhesive properties, which means it can inhibit cell adhesion, a crucial function during tissue development and repair .

Clinical and Research Implications

SPARCL1 has garnered interest in cancer research due to its down-regulation in various cancers and its potential role as a tumor suppressor . Its ability to regulate angiogenesis, the formation of new blood vessels, makes it a target for therapeutic research in oncology . Additionally, SPARCL1’s role in inhibiting cell growth has been measured in studies using mink lung epithelial cells, where it demonstrated the ability to inhibit cell growth at specific concentrations .

Recombinant Production and Applications

Recombinant mouse SPARCL1 is produced for research purposes and is available in various formulations, including carrier-free versions that do not contain Bovine Serum Albumin (BSA) . This is particularly useful for applications where the presence of BSA could interfere with experimental outcomes . The protein is typically lyophilized and can be reconstituted in sterile PBS for use in various assays .

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