SPINK6 Antibody, HRP conjugated
Description
Definition and Target Specificity
SPINK6 antibodies, HRP conjugated, are polyclonal immunoglobulin G (IgG) reagents raised against synthetic or recombinant SPINK6 peptides. These antibodies specifically bind to SPINK6, a 9.4 kDa protein that inhibits kallikrein-related peptidases (KLKs), including KLK4, KLK5, KLK6, KLK7, KLK12, KLK13, and KLK14, but not KLK8 . The HRP conjugate facilitates enzymatic detection in assays such as ELISA and immunohistochemistry (IHC).
Mechanistic Role of SPINK6
SPINK6 is a protease inhibitor critical for:
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Skin Barrier Function: Inhibits KLK5 (K<sub>i</sub> = 1.33 nM) and KLK14 (K<sub>i</sub> = 0.5 nM), modulating desquamation and inflammation .
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Viral Defense: Suppresses airway proteases (e.g., HAT, KLK5), restricting influenza A virus replication by blocking hemagglutinin cleavage .
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Therapeutic Potential: Higher SPINK6 expression correlates with protection against H7N9 infection in humans .
Inhibition Profile
SPINK6 selectively inhibits KLKs, as demonstrated by fluorogenic substrate assays :
| Enzyme | Inhibition Efficiency | Substrate |
|---|---|---|
| KLK5 | 99.9% | Tosyl-Gly-Pro-Arg pNA |
| KLK14 | 99.9% | Tosyl-Gly-Pro-Arg pNA |
| KLK7 | 88.3% | MeO-Suc-Arg-Pro-Tyr pNA |
Functional Validation
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ELISA: Detects SPINK6 in human serum and cell lysates with a sensitivity of 46.875 pg/ml .
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IHC: Localizes SPINK6 in human lung carcinoma and skin tissues at 1:50–1:300 dilutions .
Applications in Research
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Viral Pathogenesis Studies: Used to quantify SPINK6’s suppression of influenza A virus replication in airway organoids and murine models .
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Dermatology: Evaluates SPINK6’s role in skin diseases (e.g., psoriasis, eczema) via IHC .
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Protease Regulation: Measures KLK inhibition kinetics in ex vivo skin desquamation assays .
Significance and Future Directions
HRP-conjugated SPINK6 antibodies are indispensable for advancing research on protease-driven diseases and antiviral therapies. Future studies may explore:
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SPINK6’s interaction with emerging respiratory viruses.
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Clinical applications in biomarker discovery for inflammatory skin disorders.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research has demonstrated that SPINK6 expression is specifically suppressed in liver tumor tissues. This suppression can even be detected in early-stage tumors. As SPINK6 is a secretory protein, the extracellular protein level may be decreased due to tumor development. These findings support the notion that SPINK6 plays a crucial role as a tumor suppressor in the liver. PMID: 27999203
- Our research has identified a novel EGFR-activating mechanism in which SPINK6 plays a critical role in promoting nasopharyngeal carcinoma metastasis. PMID: 27671677
- Our study revealed a significant fraction of SPINK6-sensitive proteases in healthy saliva and confirmed the ability of gingipains to inactivate SPINK6 under ex vivo conditions. PMID: 27354280
- We have identified both a conserved binding mode and an alternate binding mode for SPINK inhibitors, the latter of which has not been previously observed for SPINK inhibitors. PMID: 26828269
- Our research indicates that Spink6 is a potent inhibitor of kallikrein-related peptidases and is involved in skin barrier function. PMID: 24352040
- Cross-linked SPINK6 might protect specific substrates, such as fibronectin, from kallikrein-related peptidases cleavage and contribute to the regulation of proteases in the epidermis. PMID: 23303447
- Serine protease of Kazal-type (SPINK6) expressed in normal human skin is a potent natural inhibitor of Kallikrein-related peptidases, KLK12 and KLK13. PMID: 21439340
- Data suggests that SPINK6 plays a role in modulating the activity of kallikreins in human skin. PMID: 20667819
