SPL1 Antibody

SPL1 Antibody Characteristics

SPL1 antibodies are designed for antigen-specific detection in biological samples. Key features include:

• Target: SPL1 protein (aliases: NPHS14, S1PL, SPL).

• Applications: Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), immunocytochemistry (ICC), and immunohistochemistry (IHC) .

• Tissue Expression: Ubiquitous across tissues, including liver, kidney, and immune cells .

Table 1: Common Applications of SPL1 Antibodies

Biological Roles of SPL1

SPL1 regulates sphingolipid metabolism and apoptosis, influencing fatty acid synthesis and cell survival. Its ER localization enables cross-talk with apoptotic pathways, such as caspase activation .

Diagnostic and Therapeutic Potential

While not yet widely adopted in clinical settings, SPL1 antibodies have been used to study:

• Apoptosis: SPL1 depletion triggers mitochondrial dysfunction and cell death, suggesting roles in cancer progression .

• Fatty Acid Metabolism: SPL1 modulates sphingolipid catabolism, impacting lipid homeostasis .

Table 2: Supplier and Product Overview

Note: Specific supplier names are not disclosed in publicly available data.

Antibody Validation

SPL1 antibodies are validated for specificity and sensitivity, with over 290 citations in peer-reviewed studies . Cross-reactivity with related proteins (e.g., SP1, SPLUNC1) is minimal, ensuring precise targeting .

Limitations

• Tissue Fixation: Optimal performance in FFPE (formalin-fixed, paraffin-embedded) sections requires standardized protocols.

• Batch Consistency: Recombinant antibodies (e.g., rabbit monoclonals) improve lot-to-lot reproducibility .