SPL6 Antibody
Description
Definition and Biological Context
SPL6 (SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 6) is a plant-specific transcription factor belonging to the SBP-domain protein family. It plays a critical role in regulating immune responses against pathogens, such as Tobacco mosaic virus and Pseudomonas syringae, by associating with nuclear-localized immune receptors (e.g., TIR-NB-LRR receptors) to activate defense genes . While SPL6 itself is not an antibody, research tools like SPL6-specific antibodies are essential for detecting its expression and studying its mechanisms in plant immunity.
Functional Insights from SPL6 Studies
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Role in Plant Immunity:
SPL6 associates with activated immune receptors (e.g., Arabidopsis RPS4) to regulate defense gene expression. Genetic knockout of SPL6 abolishes resistance to pathogens like TMV and Pseudomonas syringae, confirming its necessity in TIR-NB-LRR-mediated immunity . -
Mechanism of Action:
SPL6 binds to pathogen-activated receptors in the nucleus, enabling transcriptional reprogramming of defense genes. This interaction is pH-dependent and specific to effector-triggered immunity .
RPS6 Antibody (ab225676)
SP6 Antibody (Ki67)
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Clinical Utility:
Cross-Reactive Antibodies in Viral Research
While not directly linked to SPL6, broadly neutralizing antibodies like S2P6 (targeting betacoronavirus spike proteins) demonstrate methodologies relevant to antibody development:
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
Q&A
FAQs for SPL6 Antibody in Academic Research
How to resolve discrepancies in SPL6’s role across pathogen systems?
Advanced Research Focus: Context-dependent functionality.
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Pathogen-Specific Assays: Test SPL6 dependence using isogenic bacterial/fungal strains (e.g., Pseudomonas syringae AvrRps4 vs. Xanthomonas).
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Receptor Specificity: Validate SPL6’s interaction with TIR-NB-LRR receptors (e.g., RPS4) but not CC-NB-LRRs (e.g., RPS2/RPM1) via Co-IP and phenotypic assays .
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Table: SPL6 Dependency in Immune Pathways
What methodologies optimize SPL6-mediated defense gene profiling?
Advanced Research Focus: Transcriptomic analysis.
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Microarray Design: Use whole-genome arrays to compare wild-type and spl6 mutants post-pathogen challenge. Focus on genes upregulated in TIR-NB-LRR pathways .
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Time-Course Experiments: Collect samples at early (3–6 h) and late (24–48 h) infection stages to capture dynamic SPL6 activity.
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Data Validation: Combine chromatin immunoprecipitation (ChIP-seq) with RNA-seq to link SPL6 binding to promoter regions of defense genes (e.g., PR1, EDS1).
How to address low reproducibility in SPL6-antibody-based assays?
Methodological Challenge: Antibody specificity and cross-reactivity.
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Peptide Blocking: Pre-incubate SPL6 antibodies with antigenic peptides (e.g., residues 849–863 in SEZ6) to confirm signal loss in Western blot/immunofluorescence.
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Cross-Species Validation: Test antibody reactivity in multiple plant species (e.g., Arabidopsis, tobacco) using knockout controls .
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Table: Antibody Validation Checklist
What controls are critical for SPL6 interaction studies?
Basic Research Focus: Experimental rigor.
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Negative Controls:
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Positive Controls: Co-express SPL6 with known interactors (e.g., N immune receptor) in transient systems (e.g., N. benthamiana).
How to differentiate SPL6’s role in development vs. immunity?
Advanced Research Focus: Functional pleiotropy.
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Tissue-Specific Knockdown: Use CRISPR/Cas9 or RNAi in specific tissues (e.g., epidermis vs. vasculature) to isolate immune-specific phenotypes.
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Dual-Luciferase Assays: Measure SPL6’s transcriptional activity on immune-related promoters (e.g., PR1) versus developmental genes (e.g., AP1).
