SPL9 Antibody

Introduction to SPL9 Antibody

SPL9 Antibody is a rabbit polyclonal antibody raised against the C-terminal region of Squamosa Promoter-Binding Protein-Like 9 (SPL9), a plant-specific transcription factor critical for abscisic acid (ABA) signaling and cold stress responses in Arabidopsis thaliana. This antibody is designed for detecting endogenous SPL9 protein in immunological assays, enabling researchers to study its subcellular localization, post-translational modifications, and interactions with other proteins .

Applications in Research

The SPL9 Antibody is pivotal for studying SPL9’s roles in stress responses and development. Below are key research applications inferred from SPL9’s biological functions:

Protein-Interaction Studies

SPL9 interacts with ABA-responsive transcription factors (e.g., ABI5) and kinases (e.g., SnRK2s). The antibody facilitates co-immunoprecipitation (Co-IP) assays to validate these interactions .

Chromatin-Binding Assays

SPL9 binds to SBP-box motifs (GTAC) in promoters of target genes (e.g., Em6, Em1, CBF2). The antibody enables chromatin immunoprecipitation (ChIP) to map SPL9’s genomic binding sites .

Post-Translational Modification Analysis

Phosphorylation of SPL9 by SnRK2s is critical for ABA signaling. The antibody is used in immunoblotting to detect phosphorylated SPL9 isoforms .

Experimental Techniques Involving SPL9 Antibody

The antibody is validated for multiple applications, as demonstrated in SPL9-related studies:

Western Blotting

Used to detect SPL9 protein levels in transgenic lines (e.g., GFP-rSPL9) or mutants .

Co-Immunoprecipitation (Co-IP)

Identifies SPL9’s interaction partners (e.g., ABI5, SnRK2.6) by immunoprecipitating complexes .

Chromatin Immunoprecipitation (ChIP)

Maps SPL9’s binding to promoters of genes like Em6 and CBF2 .

Luciferase Complementation Imaging (LCI)

Screens for SPL9 interactions with other transcription factors (e.g., ABI5) in Nicotiana benthamiana .