SPNS2 Antibody, FITC conjugated
Description
Target Biology and Research Relevance
SPNS2 is a transmembrane transporter critical for S1P secretion, which regulates immune cell egress from lymphoid organs . Studies using Spns2-KO mice show reduced T and B lymphocyte trafficking, linking SPNS2 to autoimmune diseases and cancer metastasis . FITC-conjugated antibodies enable spatial tracking of SPNS2 expression in endothelial cells (ECs) and lymphatic tissues .
Validation and Performance
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Western Blot: Detects SPNS2 at ~60 kDa in murine Baf-3 cells and MLL-AF9 primary cell models .
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Immunohistochemistry: Localizes SPNS2 in human lung, kidney, and lymphoid tissues .
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Cross-Reactivity:
Figure 1: Example Validation Data
| Application | Dilution | Result |
|---|---|---|
| WB | 1:10,000 | Clear band at 60 kDa in human lysates |
| IHC | 1:100 | Strong endothelial cell staining |
Key Research Findings
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Lymphocyte Trafficking: SPNS2-FITC antibodies confirmed reduced T cell egress from lymph nodes (LNs) in Spns2Δ mice, correlating with attenuated experimental autoimmune encephalomyelitis (EAE) severity .
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S1P Gradient Mapping: FITC-labeled antibodies visualized SPNS2-dependent S1P gradients in LN lymphatic sinuses, critical for T cell migration .
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Therapeutic Targeting: SPNS2 inhibitors reduced lymphocyte infiltration in inflammatory models without cardiovascular side effects, validated using these antibodies .
Protocol Considerations
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Blocking: Use 5% BSA in PBS to reduce non-specific binding .
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Dilution Guidelines:
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Controls: Include Spns2-KO tissues or siRNA-treated cells to confirm specificity .
Comparative Advantages
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Our research demonstrates that Spns2 and S1P have a significant impact on proximal tubular epithelial cells. They regulate inflammatory processes, subsequent fibrotic reactions, and influence other tubular transporters involved in the pathophysiology of inflammatory and fibrotic kidney diseases. PMID: 29772789
- Butyrate and the bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development. PMID: 27562371
- In both macrophage and epithelial cell types, Spns2 has been found localized to the cytoplasm and the nucleus, aligning with a predicted bipartite Nuclear Localization Signal at the position aa282 of the human Spns2 sequence. PMID: 29112690
- Spns2 and S1P1&2 play a novel role in the activation of p47(phox) and the production of reactive oxygen species involved in hyperoxia-mediated lung injury. PMID: 27343196
- Spns2 plays key roles in regulating cellular functions in non-small cell lung cancer (NSCLC) cells. PMID: 25330231
- These findings indicate that Spns2 is required for normal maintenance of the endocochlear potential and hence for normal auditory function, and support a role for S1P signaling in hearing. PMID: 25356849
- Spns2 is an S1P transporter in vivo that plays a role in regulating not only blood S1P but also lymph node and lymph S1P levels. Consequently, it influences lymphocyte trafficking and lymphatic vessel network organization. PMID: 23180825
- The sphingosine 1-phosphate transporter, SPNS2, functions as a transporter of the phosphorylated form of the immunomodulating agent FTY720. PMID: 21084291
