Sporamin A Antibody

Introduction

Sporamin A Antibody is a polyclonal rabbit-derived antibody targeting Sporamin A, a storage protein found in sweet potato tubers. This antibody is widely used in research to detect Sporamin A in plant tissues and study its biological roles, including its antitumor properties in cancer models. Below is a detailed analysis of its characteristics, applications, and research findings.

Structure and Immunogenicity

Sporamin A is a 25-kDa protein localized in the vacuoles of sweet potato tubers, accounting for ~80% of soluble proteins. The antibody targets the 24–219 amino acid region of recombinant Ipomoea batatas Sporamin A (UniProt ID: P14715) .

3.1. Detection Methods

• ELISA: Used for quantifying Sporamin A in plant extracts .

• Western Blot: Detects native Sporamin A at 1:2000 dilution (requires fresh DTT in sample buffer) .

• Immunohistochemistry: Not reported, but inferred from antibody specificity .

3.2. Cancer Research

Sporamin A Antibody has been instrumental in studying Sporamin’s antitumor effects:

• Esophageal Squamous Cell Carcinoma (ESCC): Sporamin inhibits NF-κB DNA-binding activity and induces apoptosis via Bcl-2/Bax regulation .

• Pancreatic Cancer (PANC-1 cells): MAPK pathway activation enhances Sporamin-induced apoptosis, as shown by flow cytometry and DAPI staining .

4.1. Antitumor Mechanisms

4.2. Technical Validation

• Specificity: EMSA and supershift assays confirm NF-κB p65/p50 binding inhibition .

• Sensitivity: Detects Sporamin A at concentrations as low as 12.5 µM in ELISA .