SPRY2 Antibody
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Description
Introduction
The SPRY2 Antibody (Catalog Number: 11383-1-AP) is a polyclonal rabbit IgG antibody developed by Proteintech Group, Inc., designed to specifically target the Sprouty homolog 2 (SPRY2) protein. SPRY2 is a critical regulator of receptor tyrosine kinase (RTK) signaling pathways, modulating cellular processes such as proliferation, differentiation, and apoptosis. This antibody is broadly utilized in immunological and molecular biology research to study SPRY2's role in cancer, immune regulation, and developmental biology.
Structure and Function of SPRY2
SPRY2 belongs to the Sprouty family of proteins, which act as negative feedback regulators of RTK signaling. Its primary functions include:
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Inhibition of ERK/MAPK Pathway: SPRY2 suppresses extracellular signal-regulated kinase (ERK) activation downstream of FGF, EGF, and HGF receptors .
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Dual Role in Cancer: SPRY2 exhibits tumor suppressive or oncogenic roles depending on the cancer context. For example, it inhibits HGF-mediated signaling in leiomyosarcoma but promotes MET activation in colonic adenocarcinomas .
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Apoptosis Regulation: SPRY2 modulates cellular survival by regulating AKT and ERK pathways, influencing pro-apoptotic proteins like BAD .
Applications of SPRY2 Antibody
The antibody is validated for multiple techniques, enabling comprehensive analysis of SPRY2 expression and localization:
Western Blotting (WB)
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Detects SPRY2 in lysates of SW1990 (pancreatic cancer), Jurkat (T cell leukemia), and Neuro-2a (neuroblastoma) cells .
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Observed molecular weights align with native (35–40 kDa) and modified (60–65 kDa) forms.
Immunohistochemistry (IHC)
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Stains SPRY2 in human pancreatic cancer tissues, highlighting its association with tumor progression .
Immunofluorescence (IF/ICC)
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Visualizes SPRY2 localization in subcellular compartments, such as microtubules in unstimulated cells .
Research Findings and Publications
The SPRY2 Antibody has been instrumental in advancing research across diverse fields:
Product Specs
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. Store at -20°C. Avoid freeze-thaw cycles.
Lead Time
Typically, we can ship products within 1-3 business days after receiving your order. Delivery times may vary depending on the purchasing method and location. Please consult your local distributor for specific delivery timelines.
Synonyms
hSPRY 2 antibody; hSPRY2 antibody; MGC23039 antibody; Protein sprouty homolog 2 antibody; Sprouty 2 antibody; Sprouty homolog 2 (Drosophila) antibody; Sprouty homolog 2 antibody; Sprouty2 antibody; Spry 2 antibody; Spry-2 antibody; SPRY2 antibody; SPY2_HUMAN antibody
Target Names
SPRY2
Uniprot No.
Target Background
Function
This SPRY2 antibody serves as an antagonist of fibroblast growth factor (FGF) pathways by inhibiting FGF-mediated phosphorylation of ERK1/2. Consequently, it acts as an antagonist of FGF-induced retinal lens fiber differentiation, potentially inhibiting limb bud outgrowth and negatively modulating respiratory organogenesis. It also inhibits TGFβ-induced epithelial-to-mesenchymal transition in retinal lens epithelial cells and inhibits CBL/C-CBL-mediated EGFR ubiquitination.
Gene References Into Functions
- Overexpression of miR-592 promotes GC proliferation, migration, and invasion, and induces the EMT via the PI3K/AKT and MAPK/ERK signaling pathways by inhibiting Spry2, suggesting a potential therapeutic target for Gastric cancer. PMID: 29949784
- Nuclear Spry2 acts as a molecular link that coordinates airway and vascular growth of the cardiopulmonary system. PMID: 29409968
- Activation of ERK1/2 signaling was required for hCG-induced up-regulation of SPRY2 expression. Further, SPRY2 knockdown attenuated the AREG-induced COX-2 expression and PGE2 production by inhibiting AREG-activated ERK1/2 signaling. PMID: 27539669
- Results demonstrated that SPRY2 low-expression was significantly associated with unfavorable prognosis of gastric adenocarcinoma and that SPRY2 could inhibit FGFR2-induced ERK phosphorylation and suppress FGFR2-elicited gastric cancer cell proliferation and invasion. PMID: 28002800
- Sprouty2 inhibits amphiregulin-induced down-regulation of E-cadherin and cell invasion in human ovarian cancer cells. PMID: 27835572
- Sprouty2 (Spry2), a negative regulator of the extracellular signal regulated kinase/mitogen-activated protein kinase (Erk/MAPK) signaling pathway, was a downstream target of miR-122-5p possibly involved in regulating the keratinocyte proliferation. PMID: 27943426
- Results show the involvement of Spry2 in regulation of FosB and Runx2 genes, MAPK signaling and proliferation of mesenchymal stem cells. PMID: 28387432
- MYB acts on MAPK signaling by directly regulating transcription of the gene encoding the negative modulator SPRY2. PMID: 27748374
- ZEB1 upregulation by SPRY2 results from the combined induction of ETS1 transcription factor and the repression of microRNAs (miR-200 family, miR-150) that target ZEB1 RNA. Moreover, SPRY2 increased AKT activation by epidermal growth factor, whereas AKT and also Src inhibition reduced the induction of ZEB1. PMID: 26455323
- SPRY2 is upregulated in human CRC. Suppression of SPRY2 repressed AKT2 and EMT-inducing transcription factors and significantly increased E-cadherin expression. Concurrent downregulation of SPRY1 and SPRY2 also increased E-cadherin and suppressed mesenchymal markers in colon cancer cells. PMID: 26434583
- In cells harboring a K-Ras mutation, the serine conversion weakens the reduction of migration velocity, indicating that depending on the status of K-Ras, the serine influences Sprouty2 functions differently. PMID: 26727965
- Increased nuclear localization of p21(WAF1/CIP1) in SPRY2 downregulated colon cancer cells may explain the inhibition of cell proliferation in colon cancer cells. PMID: 26293890
- Data suggest a common mechanism by which DNA methyltransferase1 (DNMT1) and hypoxia inducible factors HIF1alpha/HIF2alpha regulate Sprouty2 (Spry2) transcription. PMID: 28196140
- We showed that Spry2 is a novel unfolded protein response target and its upregulation is dependent on PERK. PMID: 28246293
- SPRY2 has an inhibitory role in the TGFbeta signaling pathway in lens epithelial cells. PMID: 27415760
- These results establish SPRY2 as a critical negative regulator of BCR-mediated MAPK-Erk signaling in chronic lymphocytic leukemia, thereby providing one of the molecular mechanisms to explain the clinical heterogeneity of chronic lymphocytic leukemia. PMID: 26809508
- Spry2 can inhibit MM cell growth and survival with a concomitant reduction in phosphorylation of extracellular signal-regulated kinases 1 and 2 in vitro and in vivo. PMID: 27016275
- Data suggest that Spry2 acts as a scaffold to bring more pVHL/associated E3 ligase in proximity of HIF1alpha and increase its ubiquitylation and degradation. This represents a novel action for Spry2 in modulating biological processes regulated by HIFalpha subunits. PMID: 27281823
- MiR-122 could act as a tumor promoter and potentially target Sprouty2. MiR-122 promotes renal cell carcinoma cell proliferation, migration, and invasion. PMID: 28231730
- miR27b promotes the migration and invasion of gastric cancer cells via inhibition of SPRY2-mediated ERK signaling. PMID: 26781754
- Arg119Trp variant in the SPRY2 gene was identified as the probable IgA nephropathy-causing mutation. This variant is responsible for inhibition of the MAPK/ERK1/2 pathway. PMID: 25782674
- SPRY2, contrary to its roles in other cancer settings, promotes glioma cell and tumor growth and cellular resistance to targeted inhibitors of oncogenic RTKs. PMID: 25934697
- Data show that proto-oncogene protein B-raf (BRAF) inhibition induces c-Jun N-terminal kinase (c-JUN) expression and c-JUN abundance and activation by down-regulating SPRY2/4 protein expression. PMID: 26286024
- This study aimed to determine whether SPRY2 might have anti-inflammatory effects on rheumatoid arthritis fibroblast-like synoviocytes. PMID: 26265114
- miR21 may represent a negative regulator involved in the downregulation of SPRY2 in MM. PMID: 25633921
- No association between SPRY2, single-nucleotide polymorphisms, and nonsyndromic cleft lip with or without cleft palate risk were observed in this cohort of patients. PMID: 25339627
- A decrease in SPRY2 and RECK expression by nickel-induced miR-21 may promote invasiveness in lung cancer cells. PMID: 26026961
- Sprouty 2 protein, but not Sprouty 4, is an independent prognostic biomarker for human epithelial ovarian cancer. PMID: 25630587
- Results suggest a link between placental SPRY2 mRNA levels and placental growth, which may be modulated by maternal metabolic status and placental inflammation. PMID: 24969401
- This study reveals the loss of SPRY2 in HGSC and indicates an important tumor-suppressive role for SPRY2 in mediating the stimulatory effect of EGF on human EOC progression. PMID: 25533808
- CK1 activity and binding are necessary for SPRY2 inhibition of FGF-stimulated neurite outgrowth in PC12 cells. PMID: 24469046
- Data indicate that the proliferation and survival rates of HEK293T cells transfected with pDC315/hSPRY2 were significantly higher than those transfected with pDC315/EGFP (green fluorescent proteins). PMID: 24796743
- A mechanistic model of EGFR endocytosis was developed to determine the relative contributions of three parallel pathways of MIG6, ubiquitin ligase CBL, and Sprouty2. PMID: 24445374
- The ERK-MAPK signaling pathway is regulated by Sprouty2 expression via a miR-21-mediated mechanism during mesenchymal stem cell differentiation. PMID: 23239100
- SPRY2 and MIG6 are important regulators of wild-type and mutant EGFR trafficking and point to an EGFR expression-independent function of SPRY2 in the regulation of ERK activity that may impact cellular sensitivity to EGFR inhibitors. PMID: 23868981
- This study revealed the involvement of PAX9, EDA, SPRY2, SPRY4, and WNT10A as risk factors for MLIA. Three strong synergistic interactions between MLIA liability and MSX1-TGFA, AXIN2-TGFA, and SPRY2-SPRY4 gene pairs were uncovered. This is the first evidence of sprouty genes in MLIA susceptibility. PMID: 24554542
- We found that SPRY2 expression is regulated by FOXO3a and beta-catenin nuclear activity in colon cancer. PMID: 23624922
- Increased SPRY2 expression during chronic viral infection reduces T cell polyfunctionality. PMID: 24292711
- We hypothesize that the level of SPRY2 expression contributes to MAPK/ERK pathway output and accounts for BRAF V600E+ and clinical heterogeneity in papillary thyroid cancer. PMID: 24094449
- Sprouty2 (but not Sprouty4) plays a role in the inhibition of cell proliferation and migration of osteosarcoma cells. PMID: 23831057
- SPRY2 is an important regulator of branching morphogenesis and epithelial to mesenchymal transition in the mammary gland. PMID: 23573284
- Our results have raised the possibility that Spry2 might play a role in the pathogenesis and progression of renal cell carcinoma. PMID: 23688375
- Data suggest that epidermal growth factor induced the expression of c-MYC, which promoted the expression of mature miR-23a, miR-24-2, and miR-27a, subsequently decreasing the expression of Sprouty2 (SPRY2). PMID: 23649631
- miR-27a regulates MET, EGFR, and Sprouty2 in lung cancer. PMID: 23650389
- SPRY2, PTEN, and PP2A status is an important determinant of prostate cancer progression. PMID: 23434594
- In conclusion, SPRY2 is an important tumor suppressor in prostate cancer since its loss drives the PI3K/AKT pathway via functional interaction with the ErbB system. PMID: 22649008
- G Protein-regulated inducer of neurite outgrowth (GRIN) modulates Sprouty protein repression of mitogen-activated protein kinase (MAPK) activation by growth factor stimulation. PMID: 22383529
- ITSN1 binds Pro-rich regions in both Cbl and Spry2, and interaction of ITSN1 with Spry2 disrupts Spry2-Cbl interaction, resulting in enhanced ubiquitylation of the EGFR. PMID: 22158968
- A significant association of rs10906115 in CDC123/CAMK1D and rs1359790 near SPRY2 was identified with type 2 diabetes in a Japanese population. PMID: 21909839
- Analysis of regulation of cellular levels of Sprouty2 protein by prolyl hydroxylase domain and von Hippel-Lindau proteins was conducted. PMID: 22006925
Database Links
Involvement In Disease
IgA nephropathy 3 (IGAN3)
Protein Families
Sprouty family
Subcellular Location
Cytoplasm, cytoskeleton. Cell projection, ruffle membrane.
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